RESUMO
OBJECTIVE: To observe the therapeutic effect of work intensive training on workers with musculoskeletal injury(MSI). METHODS: A convenience sampling method was used to select MSI patients as research subjects. Patients were randomly divided into control group(179 cases) and experimental group(188 cases). On the basis of the conventional comprehensive rehabilitation treatment, the control group received the routine occupational rehabilitation intervention, and the experimental group received the work intensive training intervention. The intervention period was 4 weeks in both groups. We used the Post-Traumatic Stress Disorder(PTSD) Self-Rating Scale, the Fear-Avoidance Beliefs Questionnaire(FABQ) and the General Self-efficacy Scale(GSES) to assess the recovery of MSI patients. The statuses of return to work of patients were followed up by telephone after six months of discharge from hospital. RESULTS: Compared with the same group before intervention, the scores of PTSD, FABQ-P and FABQ-W in the control group and the experimental group decreased, and the score of GSES increased(P<0.01). After the intervention, the scores of PTSD, FABQ-P and FABQ-W in the experimental group were lower than those in the control group(P<0.01), and the score of GSES was higher than those in the control group(P<0.01). The rate of returning to work in the experimental group was higher than that in the control group(80.9% vs 59.2%, P<0.01). CONCLUSION: Work intensive training has curative effect on self-efficacy, physical activity, subjective pain, and post-traumatic stress disorder, and can improve the rate of resuming to work in patients with MSI.
RESUMO
<p><b>OBJECTIVE</b>To isolate and characterize endophytic fungi from seven Dendrobium species, and detect their antimicrobial activities.</p><p><b>METHOD</b>Fungal endophytes were isolated by strictly sterile sample preparation and fungal identification methods were based on their ITS ribosomal DNA (ITS rDNA gene) sequences. The agar well diffusion method was then employed to evaluate the antimicrobial activity against six pathogenic organisms and the phylogenetic tree of active isolates was constructed by the MEGA.</p><p><b>RESULT</b>Ninety-eight endophytic fungi obtained from seven Dendrobium spp., and among them twenty-four isolates, representing 11 genera and 14 species, displayed anti-microbial activities. The phylogenetic assay based on ITS-rDNA showed that 24 active isolates were sorted to 7 taxonomic orders: Hypocreales, Sordariales, Capnodiales, Eurotiales, Botryosphaeriales, Xylariales and Mucorales. The results of antimicrobial activity assay revealed that 1.02%, 10.2%, 18.4%, 1.02%, 1.02% and 10.2% of fermentation broths of 98 isolates displayed significant antimicrobial activities against E. coli, B. subtilis, S. aureus, C. albicans, C. neoformans and A. fumigatus, respectively. Four strains DL-R-3, DL-S-6, DG-R-10 and DN-S-1 displayed strong and broad antimicrobial spectrum.</p><p><b>CONCLUSION</b>Endophytic fungi associated with Dendrobium species have fungal diversity, and possess diverse antimicrobial activity.</p>