RESUMO
Water soluble extract (WSE) is an important index for the quality evaluation of Astragali Radix (AR). In this study, the WSE of the wild AR from Shanxi province (SX) and the cultivated AR from Gansu Province (GS) were compared. The WSEs of two types of AR were determined according to the appendix of Chinese pharmacopoeia. Then the WSEs were subjected to NMR analysis, and the obtained data were analyzed using HCA, PCA, OPLS-DA, microarray analysis, and Spearman rank analysis. In addition, the Pearson correlation of differential metabolites were also calculated. The results showed that the WSE content of GS-AR (37.80%) was higher than that of SX-AR (32.13%). The main constituent of WSE was sucrose, and other 18 compounds, including amino acids, organic acids, were also detected. Multivariate analysis revealed that SX-AR contained more choline, succinic acid, citric acid, glutamate, taurine and aspartate, while GS samples contained more sucrose, arginine and fumaric acid. In addition, the Pearson correlations between different metabolites of the two types of AR also showed apparent differences. The results suggested that the WSE of two types of AR differs not only in the content, but also in the chemical compositions. Thus, the cultivation way is important to the quality ofAR. This study supplied a new method for the comparison of extract of herbal drugs.
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Objective To discuss the correlative factors of fetal growth reatriction(FGR)in order to earlier detect,earlier diagnose,earlier treat FOR and prevent the complications and subsequent syndrome of FGR.Methods 292 cases of FGR were retrospectively analyzed during the last several years in our hospital.Results Among 292 cases of FGR,87(29.8%)had maternal factor,18(6.2%)had fetal factor,17(5.8%)had placental factor,58 (19.9%)had umbilical cord factor,112(38.0%)had unknown.In vagina childbirth group,newborn asphyxia was 24(41.4%),and in c-section group,there was 24(10.3%).There was significant difference between them.Conclu- sion The main cause of FGR is maternal factor.Treating the complications and subsequent syndrome of pregnance timely and rightly,broadening the refers drafts of c-section properly would be advantageous in reducing the sequelas of FGR.
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Objective To establish a method for determination of carbendazim(MBC) in fruits by using novel activated carbon fiber SMPE coupled with GC.Methods Carbendazim(MBC) in the fruits was determined by homemade activated carbon fiber-solid phase microextraction(ACF-SPME) coupled with GC-ECD.The conditions were optimized.Results The linear range was 1-100 ?g/L.The detection limit was 0.002 ?g/L.The recovery rates were 89%-95% with the relative standard deviation(RSD) was 5.6%.This method had successfully been applied to the determination of MBC in the fruits.Conclusion This method reveals a lower detection limit,high accuracy and needs no organic solvent,it is suitable for the determination of MBC in fruit.
RESUMO
Objective To study the neurotoxic effects of acute exposure of methylmercury at low dose and to provide some experimental data for deeply exploring the early mechanism of neurotoxicity of methylmercury. Methods SD rats were administered with methylmercury chloride by intraperitoneal injection with different doses of 0.05, 0.50 and 5.00 mg/kg and different exposure times of 20 min, 1 h, 4 h, 24 h. Mercury, ACh and AChE contents in rat brains were measured. Results Mercury contents in rat brains significantly increased after 20 min-exposure at both 0.50 and 5.00 mg/kg doses. Significant increase occurred after 4 h exposure at dose of 0.05 mg/kg. ACh and AChE in rat brains significantly changed after 20 min at every dose, showing certain dose-response and time-response relations. Conclusion Changes of ACh and AChE in rat brain after administration of low dose(0.05 mg/kg) and short time(20 min) exposure suggested certain modulation of CNS had been initiated. With the increases of exposure dose and time, methylmercury might begin to accumulate in rat brain and induce the significant changes of ACh and AChE.