Establishment of sorting method of CD3+CD4+T lymphocytes in human intestinal tissue / 重庆医学

Objective To establish an accurate sorting method with less interference to the cell activity for isolating CD 3+CD4+T lymphocytes in human intestinal tissue .Methods Firstly the digestive enzymes were adopted to decompose the human intestinal tissue ,and then the cell suspension was obtained by the filtration of 40 μm cell strainer .Mononuclear cells were collected by using continuous density Percoll gradient centrifugation technique ,then the flow cytometry was adopted to sort CD3+ CD4+ T lympho-cytes in mononuclear cells .The survival rate ,cell purity and morphological observation of the sorted cells were adopted to conduct the evaluation on the sorting method .Results The digestive enzymes well decomposed human intestinal tissue into cell suspension , the purity of CD3+CD4+ T lymphocytes by using continuous density Percoll gradient centrifugation was (43 .9 ± 7 .3)% ,while the purity of CD3+CD4+ T lymphocytes was(96 .9 ± 1 .2)% after fluorescence-activated cell sorting ,the difference between them was statistically significant(P<0 .01) .The survival rate of sorted CD3+CD4+ T lymphocytes was(97 .8 ± 1 .6)% with intact cell shape . Conclusion The Percoll gradient centrifugation technique combined with the fluorescence-activated cell sorting not only collects highly pure human intestinal CD3+CD4+ T lymphocytes with little influence on the cell activity and shape ,but aslo may be carried out under sterile conditions ,the sorted cells can continue to be used in the other functional researches .

The Preliminary Analysis on the Sex Difference of Patients with Primary Hepatic Cancer in Guilin Districk / 中国医师杂志

Objective To study the sex difference among PHC patients' ABO blood types and HBV infection,hepatic cancer family history,drunking alcohol hobby in Guilin district.Methods 192 cases of PHC were classified according to sexes,we compared the ABO blood types distribution,HBV infection,family history of PHC and drunking alcohol hobby between the two sexes.Results PHC infective ratio of male to female was 85.94∶14.06;the O blood type distribution in the male was more than that of the female's(P

Scanning Electron Microscopical Observations on Damages to Tegument and Gut Epithelium of Paragonimus Heterotremus by Albendazole / 昆明医科大学学报

The adult worms of Paragonimus heterotremus obtained from experimental infected rats were observed under a scanning electron microscope.24 hours after gastrogavage of albendazole 100mg/kg/d?2,the whole tegument of the worms was markedly swelled,and the posterior togument showed hollow.After 5 days the swollen tegument around the oral sucker looked like cotton fibre,the tegument around the ventral sucker showed local necrosis, and the posterior tegument still showed swollen papillae.The gut epithelium showed local necrosis and the lamella on its surface formed lumps.