Effect of piperine on 5-HT and synaptophysin expression of rats with irritable bowel syndrome / 药学学报

This study is to explore the amelioration of piperine on chronic acute combining stress rat with depression-like behavior, visceral sensitivity, and its effect on the expression of serotonin (5-HT) and synaptophysin. Forty two SD rats were divided into seven groups: blank group, model group, piperine (12.5, 25, 50 and 100 mgkg-1, ig) and imipramine (10 mgkg-1, ip) groups. The rat model of irritable bowel syndrome was established by chronic acute combining stress, and then to evaluate depression-like behavior and visceral sensitivity. The expressions of 5-HT and synaptophysin in the hippocampus and colon were determined by high performance liquid chromatography (HPLC) and Western blotting, respectively. The duration of immobility of IBS rat in the forced swimming test had been significantly increased, the sucrose consumption of IBS rat had been reduced and visceral sensitivity was obviously elevated in the IBS model group as compared with those in the normal control group (P<0.05, P<0.01). As compared with those in the normal control group, the expression of 5-HT significantly decreased, 5-HIAA/5-HT ratio significantly increased in the hippocampus of IBS model group (P<0.05), but opposite presentations were noted in the colon (P<0.05). As compared with that in the normal control group, the synaptophysin expression in the hippocampus decreased significantly but obviously increased in the colon (P<0.05). Piperine improved the behavior of IBS rats, and reversed the levels of 5-HT and 5-HIAA, and 5-HIAA/5-HT proportion in the hippocampus and colon (P<0.05); besides, they significantly reverse the synaptophysin level in the hippocampus and colon (P<0.05). The presence of depression and visceral sensitivity had been changed in IBS rats, with abnormal expression of 5-HT and synaptophysin in the brain-gut system. Piperine can ameliorate the changes of the behavior and regulation of serotonin and synaptophysin expression in IBS rat model.

Optimization of the Forming Technology of Duanxueliu Dropping Pills by Orthogonal Experiment / 中国药房

OBJECTIVE:To optimize the forming technology of Duanxueliu dropping pills.METHODS:The forming technology of Duanxueliu dropping pills was optimized by orthogonal experiment with the type of matrix,the ratio of extractum to base material and the dropping temperature as factors and with RSD(weight variance of pills),the dissolution time,molding ratio of dripping pills as indexes,meanwhile,comprehensive scoring was carried out.The dripping conditions were optimized with dropping distance,dropping speed,the internal and external diameters of the burette as factors and with variation coefficient of pill weight as index.RESULTS:The optimized parameters for the forming technology were as follows:PEG6000 was used as base material with the ratio of extractum to base material at 1∶3 and the dropping temperature at 75 ℃.The optimized dripping conditions were as follows:the dropping distance was 10 cm;the dropping speed was 50～60 drops per minute;the internal diameter of the burette was 5.0 mm and the external diameter of the burette was 3.0 mm.CONCLUSIO-N:This forming technology serves as experimental basis for the preparation of Duanxueliu dropping pills.