Simultaneous Determination of Isoquercitrin ，Astragalin and Salvianolic Acid B in Moringa oleifera Leaves Granules by HPLC / 中国药房

OBJECTIVE： To establish a method for simultaneous determination of isoquercitrin， astragalin and salvianolic acid B in Moringa oleifera leaves granules. METHODS： HPLC method was adopted. The determination was performed on Cosmosil-C18 column with mobile phase consisted of acetonitrile-0.1％ phosphoric acid solution （gradient elution） at flow rate of 1.3 mL/min. The column temperature was 40 ℃ and detection wavelength was set at 260 nm. The sample size was 10 μL. RESULTS： The linear ranges of isoquercitrin， astragalin and salvianolic acid B were 0.017-0.341， 0.010-0.194， 0.010-0.195 mg/mL， respectively （all r＞0.999 0）. The detection limits were 0.085， 0.143， 0.117 μg/mL， and the limits of quantitation were 0.283， 0.476， 0.392 μg/mL， respectively. RSDs of precision， stability （24 h） and reproducibility tests were all lower than 2.0％ （n＝6）. Average recoveries were 101.22％， 98.76％ and 98.72％， and RSDs were 0.66％，0.30％，0.30％ （n＝6）， respectively. CONCLUSIONS： The established method is simple， accurate and reproducible. It can be used for simultaneous determination of isoquercitrin， astragalin and salvianolic acid B in M. oleifera leaves granules.

Cloning and expression profile of Bmyan in the silkworm (Bombyx mori) and experimental validation as one target of microRNA 7 / 生物工程学报

microRNAs (miRNAs) are an extensive class of -22-nucleotide (nt) endogenous noncoding RNAs regulating life activities ofmetazoans through binding to 3'-untranslated regions (3'-UTRs) of their target genes. This work aimed to identify yan gene in the silkworm, reveal its expression profile and confirm if it is one target of bmo-miR-7 and, as such, have potential for contributing to better understanding of the molecular mechanisms involved in the metamorphosis of silkworm. Based on homolog searching and PCR amplification, we cloned the coding sequence (CDS) of Bmyan, which encodes 476 amino acid residues and contains SAM-PNT and ETs domains. Quantitative PCR (q-PCR), RT-PCR and microarray data revealed high expression of Bmyan in the head, body wall and ovary of day-3 fifth instar larval silkworm, low or no expression in other tissues. It was lowly expressed in the early larval stages, but highly expressed from late spinning to day 4 pupa. The 3'-UTR of Bmyan was obtained by rapid-amplification of cDNA ends (3'RACE) and predicted to contain two potential recognition sites of bmo-miR-7. The luciferase reporter vector containing the 3'-UTR of Bmyan was constructed and co-transfected into BmE cell line with the mimic of bmo-miR-7 and the decreased relative activity of luciferase showed that Bmyan is one target of bmo-miR-7. This work helps further functional analysis of bmo-miR-7 and Bmyan in the silkworm.

Value of ultrasonography in early diagnosis of metatarsal fatigue fractures / 中华超声影像学杂志

Objective To observe the ultrasonic manifestation of metatarsal fatigue fractures and probe into the clinical values of ultrasonic diagnosis and follow-up to the disease.Methods The clinical data and sonographic features of 30 cases with metatarsal fatigue fracture confirmed by X-ray and CT were analyzed retrospectively.Results All the subjects in this study were metatarsals insufficiency fractures located in the second or third diaphysis.2D-ultrasound found microfracture in the cortex and the periosteal elevation by increased vascularity in the early stage.During the 3-month follow-up,thickened cortex and callus shown as the shape of mushroom or cauliflower and a great amount of neovascularisation were visualised with color Doppler flow imaging.Then,the cortex surface became gradually changing from rough to smooth and obvious posterior shadows were observed with the illness progression.Conclusions Ultrasonography could be used to detect the cortical continuity,callus characteristics and blood flow perfusion of fatigue fracture in the different damage stages.

Effects of oxymatrine on proliferation of HepG2 cells / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the effects and the mechanisms on oxymatrine inhibiting proliferation of HepG2 cells.</p><p><b>METHOD</b>HepG2 cells were exposured to oxymatrine, final concentrations of which were 0.1, 0.2, 0.3, 0.4, 0.5, 0.8, 1, 2, 5, 8, 10, 15 g x L(-1) respectively, MTT method was used to determine the inhibiting effects of oxymatrine on HepG2 cells proliferation, followed by Haematoxylin and Eosin staining to observe the cell morphology. The expression of P53, Bcl-2 and Bax were further investigated by Immunohistochemical analysis.</p><p><b>RESULT</b>Oxymatrine below dosage of 1 g x L(-1) showed the little inhibition effect on the HepG2 cells proliferation and exhibited the significant inhibition effect from 1 to 8 g x L(-1) in both a time-and dose-dependent manner. Whereas the dosage was above 8 g x L(-1), oxymatrine didn't showed the time- and dose-dependent relationship. The results of immunohistochemical analysis indicated that the expression of Bax obviously increased and that of Bcl-2 and P53 decreased.</p><p><b>CONCLUSION</b>Oxymatrine could notably inhibit the HepG2 cells proliferation probably via upregulating the expression of the Bax and downregulating the expression of Bcl-2 and P53.</p>

Ways and effects of purchasing drugs through tender as implemented in our hospital / 中华医院管理杂志

In order to lower the purchasing prices of drugs, prevent unhealthy tendencies that might arise in the process of drug circulation in the hospital, and reduce the financial burdens of patients, our hospital started from March 1997 the practice of purchasing drugs through open tender. The measures adopted include: ①establishment of a leading group in charge of drug purchases and a drug purchasing group; ②formulation and earnest implementation of the system of purchasing drugs through open tender, making “five checks”; ③standardization of the scope of routine drugs used in the hospital; and ④adherence to the system of examination and approval by the Drug Management Committee when introduction of new drugs is being considered. Since the adoption of the system of purchasing drugs through tender, the purc hasing prices of drugs have on the average dropped 14.7% and the drug expenses for single entity diseases have been lowered.