Inhibition of mitogen-activated proliferation of human lymphocytes in vitro by high concentrations of glutamine

We invetigated the influence of hogh concentrations of glutamine and aspargine on in vitro cellular growth of lymphocytes stimulated with phytohaemaglutinin (PHA), concanavalin A (Con A) and pokeweed mitogen (PWM), a recognized test of cellular immunocompetence. Human peripheral lymphocytes were cultured in flat-bottomed 96-well microplates at 37§ C for 96 (PHA and Con A) or 144 hours (PWM) in the presence of a mitogen at different concentrations and either glutamine or asparagine supplemented at doses of 2, 4 or 8 mM. Lymphocyte reactivity, meas ured by the incorporation of tritiated thymidine into cellular DNA, was compared to identical cultures in the absence of supplemented amino acids (controls). We found that glutamine in doses of 2 mM and higher inhibited lymphocyte proliferation of mitogen-stimulated human lymphocyes, whereas asparagine caused no effect. These results demonstrate that, although necessary for cellular division in moderate amounts, glutamine in high concentrations has the reverse effect

Angioedema hereditario: revisao,apresentacao de uma familia com 15 individuos afetados e correlacao com sistema HLA. / Hereditary angioedema: review, report of a family with 15 affected subjects and correlation with the HLA system

Os autores apresentam sua experiencia com o angiodema hereditario, descrevendo diversos casos no Rio Grande do Sul. As familias acometidas foram investigadas com dosagem de C4, tipagem HLA, grupo sanguineo e Rh. Conclui-se que nao existe relacao entre o surgimento da doenca e antigenos de histocompatibilidade, grupos sanguineos ABO e Rh. A dosagem de C4 e importante na confirmacao do diagnostico clinico

Listeriose como causa de morte fetal: relato de um caso. / Listeriosis as cause of fetal death: report of a case

Apresentacao de revisao sucinta de listeriose com maior enfase na sua importancia na gestacao. Relato de caso de listeriose descoberta em paciente internada no Hospital Universitario da PUC com quadro de trabalho de parto prematuro.Quando da baixa o concepto estava vivo. O obito fetal ocorreu durante a internacao obstetrica. E descrita a rotina de investigacao sorologica e bacteriologica