RESUMO
OBJECTIVES: The purpose of this study was to investigate the effects of low-level laser therapy (LLLT) with a diode gallium-aluminum-arsenide (Ga-Al-As) low-level laser device on the healing and attachment of titanium implants in bone. MATERIALS AND METHODS: Thirteen New Zealand white male rabbits weighing 3.0+/-0.5 kg were used for this study. Dental titanium implants (3.75 mm in diameter and 8.5 mm in length, US II RBM plus fixture; Osstem, Seoul, Korea) were implanted into both femurs of each rabbit. The rabbits were randomly divided into a LLLT group and a control group. The LLLT was initiated immediately after surgery and then repeated daily for 7 consecutive days in the LLLT group. Six weeks and 12 weeks after implantation, we evaluated and compared the osseointegration of the LLLT group and control group, using histomorphometric analysis, removal torque testing, and resonance frequency analysis (RFA). The results were statistically significant when the level of probability was 0.05 or less based on a non-parametric Mann-Whitney U-test. RESULTS: The implant survival rate was about 96%. Histologically and histomorphometrically, we observed that the titanium implants were more strongly attached in LLLT group than in control group. However, there was no significant difference between the LLLT group and control group in removal torque or RFA. CONCLUSION: Histologically, LLLT might promote cell-level osseointegration of titanium implants, but there was no statistically significant effects.
Assuntos
Humanos , Masculino , Coelhos , Experimentação Animal , Densidade Óssea , Implantes Dentários , Fêmur , Terapia com Luz de Baixa Intensidade , Nova Zelândia , Osseointegração , Seul , Taxa de Sobrevida , Titânio , TorqueRESUMO
Bilateral sagittal split ramus osteotomy is considered a standard technique in mandibular orthognathic surgeries to reduce unexpected bilateral stress in the temporomandibular joints. Unilateral sagittal split ramus osteotomy (USSO) was recently introduced to correct facial asymmetry caused by asymmetric mandibular prognathism and has shown favorable outcomes. If unilateral surgery could guarantee long-term postoperative stability as well as favorable results, operation time and the incidence of postoperative complications could be reduced compared to those in bilateral surgery. This report highlights three consecutive cases with long-term follow-up in which USSO was used to correct asymmetric mandibular prognathism. Long-term postoperative changes in the condylar contour and ramus and condylar head length were analyzed using routine radiography and computed tomography. In addition, prior USSO studies were reviewed to outline clear criteria for applying this technique. In conclusion, patients showing functional-type asymmetry with predicted unilateral mandibular movement of less than 7 mm can be considered suitable candidates for USSO-based correction of asymmetric mandibular prognathism with or without maxillary arch surgeries.
Assuntos
Humanos , Assimetria Facial , Seguimentos , Cabeça , Incidência , Cirurgia Ortognática , Osteotomia Sagital do Ramo Mandibular , Complicações Pós-Operatórias , Prognatismo , Radiografia , Articulação TemporomandibularRESUMO
Assuntos
Animais , Humanos , Anestesia Geral , Azaperona , Durapatita , Osteoblastos , Osteogênese , Polietilenos , Polipropilenos , Sementes , SuínosRESUMO
0.05). Amnesia during local injection was observed in eight patients (34.8%). Compared with the preoperative anxiety score, the intraoperative anxiety score was decreased.CONCLUSION: In this study, we found cardiovascular and respiratory stability in intravenous sedation using dexmedetomidine with pethidine, in plate and screw removal, after orthognathic surgery. Furthemore, intravenous sedation using dexmedetomidine with pethidine shows adequate analgesic and sedative effects.
Assuntos
Humanos , Amnésia , Ansiedade , Pressão Arterial , Pressão Sanguínea , Dexmedetomidina , Frequência Cardíaca , Hipnóticos e Sedativos , Meperidina , Cirurgia Ortognática , OxigênioRESUMO
Assuntos
Osso e Ossos , Carbocianinas , Técnicas de Cultura de Células , Técnicas de Cocultura , Colágeno , Colagenases , Combinação de Medicamentos , Durapatita , Células Endoteliais , Laminina , Lipoproteínas , Magnetismo , Imãs , Mandíbula , Células-Tronco Mesenquimais , Dente Serotino , Nylons , Osteoblastos , Osteogênese , Percloratos , Periósteo , ProteoglicanasRESUMO
0.05).CONCLUSION: The angle between the Lo line and IP line (angle of the Lo-IP line) showed no statistically significant difference in both the control and asymmetry groups. Therefore, the Lo line could be used as a horizontal reference plane in CBCT generated PA cephalograms.
Assuntos
Humanos , Tomografia Computadorizada de Feixe Cônico , Assimetria FacialRESUMO
Assuntos
Humanos , Colágeno , Combinação de Medicamentos , Durapatita , Células Endoteliais , Laminina , ProteoglicanasRESUMO
Assuntos
Adulto , Humanos , Tecido Adiposo , Ácido Ascórbico , Desenvolvimento Ósseo , Medula Óssea , Técnicas de Cultura de Células , Separação Celular , Colágeno , Colagenases , Combinação de Medicamentos , Durapatita , Células Endoteliais , Fator de Crescimento Epidérmico , Citometria de Fluxo , Heparina , Hidrocortisona , Laminina , Magnetismo , Imãs , Células-Tronco Mesenquimais , Microesferas , Nylons , Osteoblastos , Osteotomia Sagital do Ramo Mandibular , Prognatismo , Proteoglicanas , Pele , Células-Tronco , Fator A de Crescimento do Endotélio VascularRESUMO
Assuntos
Humanos , Artérias , Cicatriz , Estética , Antebraço , Retalhos de Tecido Biológico , Mãos , Força da Mão , Cabeça , Veias Jugulares , Maxila , Boca , Soalho Bucal , Mucosa Bucal , Pescoço , Palato , Nutrição Parenteral , Estudos Retrospectivos , Sensação , Pele , Cirurgia Bucal , Taxa de Sobrevida , Coxa da Perna , Glândula Tireoide , Doadores de Tecidos , Língua , Transplantes , PunhoRESUMO
Assuntos
Humanos , Fosfatase Alcalina , Ácido Ascórbico , Cálcio , Técnicas de Cultura de Células , Proliferação de Células , Dexametasona , Durapatita , Mandíbula , Dente Serotino , Osteoblastos , Osteocalcina , Fenótipo , RNA Mensageiro , EstrôncioRESUMO
Assuntos
Humanos , Assimetria Facial , Incisivo , Mandíbula , Osteotomia Sagital do Ramo Mandibular , Prognatismo , Recidiva , Estudos RetrospectivosRESUMO
Assuntos
Feminino , Humanos , Queixo , Assimetria Facial , Ossos Faciais , Cabeça , Hipertrofia , Má Oclusão , Côndilo Mandibular , Mordida Aberta , Cirurgia Ortognática , Osteocondroma , Músculos Pterigoides , Esqueleto , GreveRESUMO
A melanotic neuroectodermal tumor of infancy (MNTI) is a uncommon osteolytic pigmented neoplasm that primarily affects the jaws of newborn infants. Most patients (> 90%) present with the tumor in the first year of life. Approximately 65% form in the maxilla, 11% in the mandible, 5% in the brain and elsewhere. MNTI is normally benign, but up to 15% may recur and a few have metastasized. Approximately 200 cases of MNTI have been reported but only 2 of them presented as multifocal. A case of MNTI in a 7 month old boy was encountered. The chief complaint was maxillary anterior ridge swelling. The incisional biopsy findings were MNTI. Two months after the first operation, mild swelling of another site was observed. The infant was examined periodically since undergoing two procedures with no recurrence. This case demonstrates the possibility of a multicentric MNTI. We report a multicentric MNTI with a review of the relevant literature.
Assuntos
Humanos , Lactente , Recém-Nascido , Biópsia , Encéfalo , Arcada Osseodentária , Mandíbula , Maxila , Tumor Neuroectodérmico Melanótico , Polienos , RecidivaRESUMO
INTRODUCTION: Skeletal homeostasis is normally maintained by the stability between bone formation by osteoblasts and bone resorption by osteoclasts. However, the correlation between the inflammatory reaction and osteoblastic differentiation of cultured osteoprogenitor cells has not been fully investigated. This study examined the effects of inflammatory cytokines on the osteoblastic differentiation of cultured human periosteal-derived cells. MATERIALS AND METHODS: Periosteal-derived cells were obtained from the mandibular periosteum and introduced into the cell culture. After passage 3, the periosteal-derived cells were further cultured in an osteogenic induction Dulbecco's modified Eagle's medium (DMEM) medium containing dexamethasone, ascorbic acid, and beta-glycerophosphate. In this culture medium, tumor necrosis factor (TNF)-alpha with different concentrations (0.1, 1, and 10 ng/mL) or interleukin (IL)-1beta with different concentrations (0.01, 0.1, and 1 ng/mL) were added. RESULTS: Both TNF-alpha and IL-1beta stimulated alkaline phosphatase (ALP) expression in the periosteal-derived cells. TNF-alpha and IL-1beta increased the level of ALP expression in a dose-dependent manner. Both TNF-alpha and IL-1beta also increased the level of alizarin red S staining in a dose-dependent manner during osteoblastic differentiation of cultured human periosteal-derived cells. CONCLUSION: These results suggest that inflammatory cytokines TNF-alpha and IL-1beta can stimulate the osteoblastic activity of cultured human periosteal-derived cells.
Assuntos
Humanos , Fosfatase Alcalina , Antraquinonas , Ácido Ascórbico , Reabsorção Óssea , Técnicas de Cultura de Células , Citocinas , Dexametasona , Durapatita , Glicerofosfatos , Homeostase , Interleucinas , Osteoblastos , Osteoclastos , Osteogênese , Periósteo , Fator de Necrose Tumoral alfaRESUMO
INTRODUCTION: The first aim of this study was to isolate the dental tissue-derived stem cells from the dental follicle (DF), dental pulp (DP), and root apical papilla (RAP) of the extracted wisdom teeth. Second was to evaluate their characterization with the expressions of transcription factors and cell surface markers. Finally, their ability of the in vitro multi-lineage differentiations into osteogenic and adipogenic cells were compared, respectively. MATERIALS AND METHODS: Dental tissues, including dental follicle, dental pulp, and root apical papilla, were separated in the extracted wisdom teeth. These three dental tissues were cultured in Dulbecco's modified Eagle's medium (DMEM) with supplements, respectively. After passage 3, the homogeneous shaped dental tissue-derived cells were analyzed the expression of transcription factors (Oct-4, Nanog and Sox-2) and cell surface markers (CD44, CD90 and CD105) with reverse transcription polymerase chain reaction (RT-PCR) and fluorescence-activated cell sorting (FACS) analysis. In order to evaluate in vitro multi-lineage differentiations, the culture media were changed to the osteogenic and adipogenic induction mediums when the dental tissue-derived cells reached to passage 3. The characteristics of these three dental tissue-derived cells were compared with immunohistochemistry. RESULTS: During primary culture, heterogenous and colony formatted dental tissue-derived cells were observed in the culture plates. After passage 2 or 3, homogenous spindle-like cells were observed in all culture plates. Transcription factors and mesenchymal stem cell markers were positively observed in all three types of dental tissue-derived cells. However, the quantity of expressed transcription factors was most large in RAP-derived cells. In all three types of dental tissue-derived cells, osteogenic and adipogenic differentiations were observed after treatment of specific induction media. In vitro adipogenic differentiation was similar among these three types of cells. In vitro osteogenic differentiation was most strongly and frequently observed in the RAP-derived cells, whereas rarely osteogenic differentiation was observed in the DP-derived cells. CONCLUSION: These findings suggest that three types of human dental tissue-derived cells from extracted wisdom teeth were multipotent mesenchymal stem cells, have the properties of multi-lineage differentiations. Especially, stem cells from root apical papilla (SCAP) have much advantage in osteogenic differentiation, whereas dental follicle cells (DFCs) have a characteristic of easy adipogenic differentiation.
Assuntos
Humanos , Meios de Cultura , Polpa Dentária , Saco Dentário , Durapatita , Citometria de Fluxo , Imidazóis , Imuno-Histoquímica , Células-Tronco Mesenquimais , Dente Serotino , Nitrocompostos , Reação em Cadeia da Polimerase , Transcrição Reversa , Células-Tronco , Fatores de TranscriçãoRESUMO
Assuntos
Humanos , Osso e Ossos , Cálcio , Durapatita , Osteoblastos , Osteocalcina , Polietilenos , Polímeros , Polipropilenos , RNA Mensageiro , Engenharia Tecidual , TransplantesRESUMO
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Humanos , Fosfatase Alcalina , Cálcio , Técnicas de Cultura de Células , Proliferação de Células , Dexametasona , Durapatita , Glucocorticoides , Osteoblastos , Osteogênese , Osteonecrose , Osteoporose , Periósteo , FenótipoRESUMO
Assuntos
Adulto , Animais , Humanos , Regeneração Óssea , Transplante Ósseo , Durapatita , Orelha , Fibrina , Adesivo Tecidual de Fibrina , Minerais , Osteocalcina , Osteogênese , Pele , Suínos , TransplantesRESUMO