RESUMO
Grapefruit seed extract has been reported to have antimicrobial effect. The purpose of this study was to evaluate the antimicrobial and anti-gingivitis effect of chewing gum containing grapefruit seed extract and xylitol. 40 healthy subjects with gingivitis or early periodontitis were divided into two groups. Subjects in the experimental group chewed gum containing grapefruit seed extract and xylitol while subjects in the control group chewed gum containing only xylitol. All subjects received scaling and tooth brushing instruction. 1 week after scaling was set as baseline. Gingival index and plaque index were scored at baseline, 1 week, 2 week, 3 week and 4 week. Bleeding index, probing pocket depth and clinical attachment level were scored at baseline, 2 week and 4 week. The number of total bacteria and Streptococcus mutans in unstimulated saliva of experimental group were counted at 1 week, 2 week, 3 week and 4 week. Gingival indices of experimental group and control group at baseline, 1 week, 2 week, 3 week and 4 week were 0.850+/-0.298, 0.575+/-0.345, 0.533+/-0.332, 0.459+/-0.311, 0.408+/-0.224 and 0.758+/-0.379, 0.592+/-0.296, 0.563+/-0.281, 0.454+/-0.194, 0.413+/-0.199 (mean+/-SD), respectively. Plaque indices of experimental group and control group at baseline, 1 week, 2 week, 3 week and 4 week were 0.497+/-0.500, 0.375+/-0.484, 0.332+/-0.471, 0.286+/-0.452, 0.210+/-0.407 and 0.411+/-0.492, 0.375+/-0.484, 0.354+/-0.479, 0.313+/-0.463, 0.193+/-0.395, respectively. Bleeding indices of experimental group and control group at baseline, 2 week and 4 week were 0.377+/-0.177, 0.298+/-0.152, 0.192+/-0.108 and 0.383+/-0.124, 0.318+/-0.153, 0.225+/-0.126, respectively. Probing pocket depth of experimental group and control group at baseline, 2 week and 4 week were 2.56+/-1.00, 2.40+/-0.65, 2.23+/-0.64 and 2.45+/-0.68, 2.37+/-0.57, 2.19+/-0.57, respectively. Clinical attachment level of experimental group and control group at baseline, 2 week and 4 week were 2.58+/-1.01, 2.43+/-0.67, 2.26+/-0.65 and 2.49+/-0.70, 2.40+/-0.59, 2.22+/-0.62, respectively. The % of reduction of total bacteria in saliva of experimental group at 2 week, 3 week and 4 week were 46 +/- 53%, 53 +/- 5% and 69 +/- 33%. The % of reduction of Streptococcus mutans count in saliva of experimental group at 2 week, 3 week and 4 week were 52 +/- 69%, 88 +/- 30% and 89 +/- 17%. From these findings, it can be concluded that regular use of grapefruit seed extract /xylitol chewing gum may be effective to control and prevent gingivitis and may have caries-preventive effect.
RESUMO
OBJECTIVE: Degradation of the extracellular matrix and the basement membrane is believed to be associated with tumor invasion and metastasis. To evaluate the roles of Matrix metalloproteinase-2 (MMP-2) in cervical cancer and its utility as a potential diagnostic tumor biomarker, we investigated the expression of MMP-2 in cervical cancer tissue and sera. METHODS: Through the zymography of MMP-2 in cervical cancer tissues from 35 patients, we investigated the extent and distribution of MMP-2. Also, up-regulation of its gene expression was examined by reverse transcription-polymerase chain reaction (RT-PCR) analysis. Then, we attempted to investigate by ELISA serologic responses in the sera obtained from 35 cervical cancer patients, and examined its utility as diagnostic and prognostic tumor marker in cervical cancer by comparing it with conventional tumor markers SCCA and CEA. RESULTS: In zymography, the active form of MMP-2 was detected in 66kDa. In cervical cancer the active form of MMP-2 is more strongly reacted with the substrates than those in normal tissues. Also, the mRNA of MMP-2 was more up-regulated in cervical cancer tissue than in normal one. MMP-2 was detected in the sera of cervical cancer patients, and its amount differed according to clinical situations. While it was not more effective than previous tumor markers SCCA and CEA in diagnosis, it was more useful in monitoring of the cancer. The effect was especially remarkable when it was combined with any other conventional tumor markers. CONCLUSIONS: The results suggest that the expressions of MMP-2 correlate with cervical cancer invasion and metastasis. Also, it may be useful in the monitoring of the cervical cancer lesion.
Assuntos
Humanos , Membrana Basal , Diagnóstico , Ensaio de Imunoadsorção Enzimática , Matriz Extracelular , Expressão Gênica , Metaloproteinase 2 da Matriz , Metástase Neoplásica , RNA Mensageiro , Biomarcadores Tumorais , Regulação para Cima , Neoplasias do Colo do ÚteroRESUMO
A case of malignant melanoma is described. The patient, 67 years old male, had thumb tip sized two tumor masses with linear arcuate spread on left upper back near spine of 3 months duration, and metastatic lesion on right axillary lymphnode. Lesions were removed surgically and split skin graft was done.
Assuntos
Idoso , Humanos , Masculino , Melanoma , Pele , Coluna Vertebral , Polegar , TransplantesRESUMO
The one case of sparganosis is described, the patient, 45 years old male, had adult fist sized tumor mass on scrotum for 3 year's duration and removed 4 adults sparganum mansonoides by surgical operation. Attention is calIed to the importance of sparganosis in subcutaneous swelling or tumor because of to took lived snake or non-boiling water in customary at Korea.
Assuntos
Adulto , Humanos , Masculino , Pessoa de Meia-Idade , Coreia (Geográfico) , Escroto , Serpentes , Esparganose , Plerocercoide , ÁguaRESUMO
The one case of sparganosis is described, the patient, 45 years old male, had adult fist sized tumor mass on scrotum for 3 year's duration and removed 4 adults sparganum mansonoides by surgical operation. Attention is calIed to the importance of sparganosis in subcutaneous swelling or tumor because of to took lived snake or non-boiling water in customary at Korea.
Assuntos
Adulto , Humanos , Masculino , Pessoa de Meia-Idade , Coreia (Geográfico) , Escroto , Serpentes , Esparganose , Plerocercoide , ÁguaRESUMO
Two cases of xeroderma pigmentosum in brothers without complicated by malignant changes were presented. The clinical and histopathological studies revealed characteristic features of the disease and literatures reviewed.
Assuntos
Humanos , Ictiose , Irmãos , Xeroderma PigmentosoRESUMO
Two cases of xeroderma pigmentosum in brothers without complicated by malignant changes were presented. The clinical and histopathological studies revealed characteristic features of the disease and literatures reviewed.