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Rev. Inst. Med. Trop. Säo Paulo ; 43(5): 251-255, Sept.-Oct. 2001. ilus, tab
Artigo em Inglês | LILACS | ID: lil-307997

RESUMO

Dogs sera samples collected from Cotia County, Säo Paulo were tested using indirect immunoenzymatic test (ELISA) in order to study Lyme disease serology in dogs. ELISA method was standardized and G39/40 North American strain of Borrelia burgdorferi was used as antigen. Positive results were confirmed employing the Western blotting technique. Because of the possibility of cross-reactions, sera were also tested for different serological strains of Leptospira interrogans and L. biflexa using microscopic sera agglutination test. Twenty-three of 237 (9.7 percent) serum samples were positive in the ELISA; 20 of them (86.9 percent) were confirmed by the Western blotting, what suggests that Cotia may be a risk area for Lyme disease. Although 4 samples (1.7 percent) were positive for Lyme disease and leptospirosis, no correlation was found between the results (X² = 0.725; p = 0.394) what suggests absence of serological cross reactivity


Assuntos
Animais , Cães , Masculino , Feminino , Borrelia burgdorferi , Doenças do Cão , Doença de Lyme , Anticorpos Antibacterianos , Western Blotting , Brasil , Distribuição de Qui-Quadrado , Doenças do Cão , Ensaio de Imunoadsorção Enzimática , Doença de Lyme , Estudos Soroepidemiológicos
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