RESUMO
Objective To explore effects of modified acidic fibroblast growth factor (MaFGF) on the growth of hepatocytes cultured in vitro.Methods Hepatocytes of SD rats were isolated,cultured in vitro and subculture for three generations.After being identified,the cells were used in the experiment:① Hepatocytes was observed by inverted phase contrast microscope;② The experiment were randomly divided into control and experimental group,MaFGF with different concentration were added into the culture medium of hepatocytes,after 24,48,72 h,the mitogenic effects were measured by the MTT test;③ The MTT test was used to detect the effects of MaFGF on the proliferation of the hepatocytes in 24 h.The growth curve was plotted.Results ① MaFGF potentiated the proliferation of hepatocytes,which was no significant effect with dose increased.The proliferative rate of 24 h were higher than that of the other time points (P ＜ 0.05).there was statistical difference between the MaFGF group (4.68 × 10-3 ～ 7.80 × 10-3 mg/L) and the control group (P ＜0.05).However,the mitogeoic activities of each MaFGF group were insignificant;② Four days after MaFGF(6.24 × 10-3 mg/L) added into the medium,the population of the hepatocytes was larger than that of the control (P ＜ 0.05).The number of the hepatocytes incubated with MaFGF (12.4 × 104) was 2.1 times as large as the control(6 × 104) in the 10th day(P ＜ 0.05).Conclusion MaFGF has a certain role in promoting the proliferation of the hepatocytes at the appropriate concentration and time.However,the effects don't have concentration deoendent manner.