RESUMO
The course of all the ontogenesis in cattle is closely connected with the dynamics of blood system indices which are largely integrated in the animals’ bodies. Interest to peculiarities of platelet activity in high-productive cows increases because of its great signification for the optimum of microcirculation, anabolism and maximal productivity. The aim of the research: to study peculiarities of platelet activity in healthy Ireshire heifers being on supplementary breeding. We examined 36 Ireshire heifers at the age of 12, 15 and 18 months which were on supplementary breeding. We applied hematological and statistical methods of investigation. The most active platelets’ aggregation was noted in response to adenosine diphosphate being maximal to the end of observation. Collagenic and ristomicinic aggregation had less evidence and similar directivity. Summarizing index value of platelets’ aggregation with three used inductors was equal to the end of observation to 25.6±0.05%, 10.0±0.26% and 10.3±0.39%, respectively. It indirectly pointed at high integrity of vessels’ endothelium in the animals and little concentration of von Willebrand’s Factor in their plasma. Disaggregative platelets’ activity in respect of all the applied inductors had an upward trend in the observed heifers counterbalancing the rise of aggregation. Detected low platelets’ activity in Ireshire heifers should be considered as the necessary condition for optimal provision of anabolic processes in their growing tissues and of the basis for their future high productivity. Received results can be used in future researches of Ireshire heifers as normative values.
RESUMO
This study aims to explore the effect of microRNA-21 (miR-21) on the proliferation of human degenerated nucleus pulposus (NP) by targeting programmed cell death 4 (PDCD4) tumor suppressor. NP tissues were collected from 20 intervertebral disc degeneration (IDD) patients, and from 5 patients with traumatic spine fracture. MiR-21 expressions were tested. NP cells from IDD patients were collected and divided into blank control group, negative control group (transfected with miR-21 negative sequences), miR-21 inhibitor group (transfected with miR-21 inhibitors), miR-21 mimics group (transfected with miR-21 mimics) and PDCD4 siRNA group (transfected with PDCD4 siRNAs). Cell growth was estimated by Cell Counting Kit-8; PDCD4, MMP-2,MMP-9 mRNA expressions were evaluated by qRT-PCR; PDCD4, c-Jun and p-c-Jun expressions were tested using western blot. In IDD patients, the expressions of miR-21 and PDCD4 mRNA were respectively elevated and decreased (both P<0.05). The miR-21 expressions were positively correlated with Pfirrmann grades, but negatively correlated with PDCD4 mRNA (both P<0.001). In miR-21 inhibitor group, cell growth, MMP-2 and MMP-9 mRNA expressions, and p-c-Jun protein expressions were significantly lower, while PDCD4 mRNA and protein expressions were higher than the other groups (all P<0.05). These expressions in the PDCD4 siRNA and miR-21 mimics groups was inverted compared to that in the miR-21 inhibitor group (all P<0.05). MiR-21 could promote the proliferation of human degenerated NP cells by targeting PDCD4, increasing phosphorylation of c-Jun protein, and activating AP-1-dependent transcription of MMPs, indicating that miR-21 may be a crucial biomarker in the pathogenesis of IDD.