Progress of Research on Combination of Intratumoral Immune Injection and Radiotherapy for Tumor / 肿瘤防治研究

With the rapid development of tumor immunotherapy in recent years, therapeutic cancer vaccines are attracting increased attention. Compared with personalized neoantigen vaccines, in situ vaccines could form an antigen reservoir in the tumor itself. Subsequently, antitumor immunity is initiated and the response to immune-checkpoint inhibitors of some patients improve without necessitating these patients to undergo the complicated procedures of detecting personalized antigen and customizing and synthesizing antigen peptide. At this stage, the potential of realizing the clinical translation of in situ vaccination is tremendous. In this review, we primarily introduce the mechanisms of radiotherapy and intratumoral immune injection as in situ vaccination and discuss the current status of preclinical study and clinical application of their combination to attract more attention from researchers and clinicians toward in situ vaccination.

Construction of PC9 cell lines stably transfectedwith human LncRNAMIR31HG gene and its effects on cell proliferation and migration / 吉林大学学报(医学版)

Objective: To detect the effect of over expressed human MIR31HG gene on the proliferation and m grat on of PC9 ce Is, and to clarify the mechan sm of oncogene MIR31 HG Methods: The ful length sequence of ong non coding RNA (LncRNA) MIR31HG was amp fed by RT PGR and cloned nto the pcDNA3 1 ( ) eukaryot c expression vector. The PG9 cells were transfected w th the pcDNA3 1 MIR31HG overexpress on vector and control vector pcDNA3 1. The construction of MIR31HG overexpress on vector was detected by enzymatic digest on identification. The stab e cell 1 nes w th overexpress on of MIR31HG (PC9 pcDNA3 1 MIR31HG∗ stab e transfection group) and control ce 1 1 nes (PC9 pcDNA3 1, empty vector group) were established by G418 drug screen ng. and the express on evel of MIR31 HG gene n stably transfected cell ine was detected by RT PGR CCK 8 method and scratch heal ng assay were used to detect the proliferation act vities and m gration ab 1 ties of PC9 eel s. Results: The agarose gel e ectrophoresis resu ts showed that the spec f c gene fragment of MIR31HG was obta ned by amplif cation successfu ly. The gene fragments of target gene and vector were produced by double enzyme digest on of MIR31HG eukaryotic expression vector. The RT PGR resu ts showed that the MIR31HG RNA expression leve in the cells n stable transfect on group was sign ficantly h gher than that in empty vector group (P< 0 05). The results of CGK 8 test showed that the pro iferat on act vities of the eels in stable transfection group were s gnif cant y higher than those in empty vector group at 24, 36 and 48 h after culture ( P<0 01). The results of scratch healing assay showed that the scratch heal ng rate of ce Is n stable transfect on group was sign ficantly h gher than that in empty vector group at 48 h after culture ( P<0 05). Conclusion: The eukaryotic overexpression vector and the PG9 eel line stably transfected w th human LncRNA MIR31 HG gene are constructed successfully, and M1R31 HG overexpression can promote the pro iferat on and m grat on of ung cancer PG9 cells.

Progress of pancreatitis-cancer transformation / 肿瘤研究与临床

Pancreatitis is one of the important risk factors in the occurrence of pancreatic neoplasms. Several inflammation-related transcription factors and pathways have been confirmed to be involved in the carcinogenesis, but molecular mechanisms of pancreatitis-cancer transformation are still unclear, which largely limits the further development of potential anti-tumor drugs. Thus, it is important to figure out the underlying molecular mechanisms of pancreatitis-cancer transformation. This paper reviews the main biological characteristics, relevant important signaling pathways, clinical intervention strategies of pancreatitis-cancer transformation.