RESUMO
Background: One determining factor of a successful in vitro fertilization [IVF] cycle is embryo quality. The aim of the present study was to evaluate associations of embryo quality and reserve markers like age, FSH and AMH
Materials and Methods: In this prospective study, 120 infertile women, aged 21-44 years, undergoing routine exploration during an unstimulated cycle preceding assisted reproductive technology [ART] at our center were studied prospectively, from February 2011 to December 2014. Descriptive parameters and patient characteristics were reported as mean [SD] or median [range] depending on the distribution. Student's t test was performed for continuous variables, Wilcoxon and Pearson's Test were used for not distributed variables and Fisher's Test was performed for categorical variables. P<0.05 was considered statistically significant
Results: Overall, at the time of investigation, patients had a mean age of 33.03 +/- 4.15 years old. On cycle day three, serum anti-Mullerian hormone [AMH] level was 3.50 +/- 1.54 ng/mL, serum follicle-stimulating hormone [FSH] level was 6.29 +/- 1.53 mUI/ mL, at baseline, women had 16.57 +/- 7.0 antral follicles. The mean of collected oocytes was 11.80 +/- 5.25, embryo I+II was 2.46 +/- 2.11. A greater number of embryos I+II was observed in young patients. By evaluating 120 patients, a significant relationship was observed between age and FSH [r=0.24, P=0.01], age with AMH [r=-0.22, P=0.02], age with collected oocytes [r=-0.23, P=0.03] and age with embryo I+II [r=-0.22, P=0.03]. A significant relationship was also observed between antral follicle count [AFC] and AMH [r=0.29, P=0.01], AFC and the number of transferred embryo [r=-0.18, P=0.03], AFC and total dose of the drugs [r=-0.23, P=0.03]. Significant relationship of FSH with total dose of drugs [r=0.19, P=0.02] was also observed. In addition, we determined significant relationships between AMH and the number of collected oocytes [r=0.38, P=0.01], AMH and the number of metaphase II oocytes [r= 0.35, P=0.01], AMH and the number of embryo [r=0.19, P=0.04] as well as AMH and total dose of the drugs [r=-0.25, P=0.01]
Conclusion: Commonly used clinical markers of ovarian reserve are reflection of the ovarian reserve, while the outcome measurements of ART and age are the best predictors of embryo quality