RESUMO
<p><b>OBJECTIVE</b>To establish determination methods of eotaxin mRNA and TNF-alpha mRNA expression in the lung tissue of mice.</p><p><b>METHODS</b>Eotaxin mRNA and TNF-alpha mRNA expressions were determined by semi-quantitative RT-PCR. The functional implications of eotaxin mRNA and TNF-alpha mRNA expression were examined by detecting the numbers of total leucocytes and eosinophils in bronchoalveolar lavage fluid(BALF).</p><p><b>RESULT</b>Eotaxin mRNA and TNF-alpha mRNA expression in lung tissue total numbers of leucocyte and numbers of eosinophil in BALF increased in sensitized mice compared with those in normal mice. Dexamethasone significantly but did not inhibit eotaxin mRNA and TNF-alpha mRNA expressions, and eosinophil infiltration in the lungs of the sensitized mice. A compound preparation of traditional Chinese medicine inhibited eotaxin mRNA and eosinophil infiltration, influenced TNF-alpha mRNA expression.</p><p><b>CONCLUSION</b>Increased eotaxin mRNA expression in lung tissue is associated with eosinophil infiltration in BALF, which indicates that the methods of semi-quantitative RT-PCR may be useful to the study of the mechanism of antiasthmatic medicine.</p>
Assuntos
Animais , Feminino , Masculino , Camundongos , Anti-Inflamatórios , Farmacologia , Asma , Tratamento Farmacológico , Metabolismo , Líquido da Lavagem Broncoalveolar , Biologia Celular , Quimiocina CCL11 , Quimiocinas CC , Genética , Dexametasona , Farmacologia , Modelos Animais de Doenças , Eosinófilos , Fisiologia , Contagem de Leucócitos , Pulmão , Metabolismo , Camundongos Endogâmicos ICR , RNA Mensageiro , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Fator de Necrose Tumoral alfa , GenéticaRESUMO
<p><b>OBJECTIVE</b>To investigate antigen-induced changes of leukotriene B(4)(LTB(4))content and LTA(4)-hydrolase mRNA expression in lung tissue and cerebral cortex in sensitized rats.</p><p><b>METHODS</b>The contents of LTB(4) in lung tissue and cerebral cortex homogenates and LTA(4)-hydrolase mRNA expression after antigen challenge by aerosol were respectively detected by reverse-phase high performance liquid chromatography(RP-HPLC) and semi-quantitative RT-PCR.</p><p><b>RESULT</b>The LTB(4) levels in lung tissue and cerebral cortex homogenates in asthmatic rats were significantly higher than those in control (P%0.05), and LTA4-hydrolase mRNA expression was also increased in asthmatic group. Dexamethason(DXM, 0.5 mg/kg, i.p.) decreased the LTB(4) content and inhibited the LTA(4)-hydrolase mRNA expression significantly in asthmatic rats(P%0.05).</p><p><b>CONCLUSION</b>LTB(4) content and LTA(4)-hydrolase mRNA expression in cerebral cortex and lung tissue are increased in asthmatic rats, and there may exist neuroimmunological cross-talking between central nervous system and lung tissue in asthma.</p>