Advances in the study of microRNA in deep vein thrombosis / 医学研究生学报

Deep vein thrombosis(DVT) refers to a blood clot that forms in a deep vein, and usually occurs in the lower extremities.Typical symptoms include swelling, pain or tenderness in the leg.DVT is one of the common complications of orthopedic surgery and has a complicated pathogenesis.The embolus can fall off into the lung, resulting in the death.MicroRNA (miRNA) is a class of endogenous and non-coding single stranded RNA consisting of 21~23 nucleotides, widely involved in a variety of biological processes.Recent studies have found that microRNA (miRNA) plays a significant role in the pathogenesis of DVT.This article reviews recent advances in the study of miRNAs as novel regulators, diagnostic tools and a therapeutic choice in DVT to provide new insights of its pathogenesis and therapeutic target.

Immune regulation of interleukin-23 on peripheral blood natural killer cell in patients with inflammatory bowel disease / 中华消化杂志

Objective To study the potential role of interleukin-23(IL-23)in induction of peripheral blood natural killer(NK)cell activation and cytotoxicity in patients with inflammatory bowel disease(IBD),and to investigate its clinical relevance to the pathogenesis.Methods Peripheral blood was collected from 12 healthy subjects,16 patients with Crohn's disease(CD)and 25 patients with ulcerative colitis(UC).NK cells isolated from peripheral blood using immunomagnetie beads were cultured in vitro and stimulated with IL-23 or IgG,or combination of two.Concentrations of tumor necrosis factor α(TNF-α)and interferon-γ(IFN-γ)were determined by enzyme linked immunosorbent assay.NK eytotoxicity to K562 cell was detected by flow cytometry.Results The levels of TNF-α and IFN-γ were not increased in healthy controls under stimulation with IgG or IL-23 (P＞0.05),but they were elevated under stimulation with IgG plus IL-23(P＜0.05).IL-23,not IgG,was found tO enhance TNF-α and IFN-γ secretion in IBD patients(P＜0.05).The levels of TNF-α and IFN-γ were significantly increased in IBD patients under the stimulation of IL-23 plus lgG (P＜0.01).The cytotoxicity of NK cells in healthy controls was not observed under stimulation with IL-23 alone or IL-23 plus IgG,whereas it could be enhanced by stimulation with IL-23 in CD patients (62.4%±5.5%)and UC patients(65.3%±6.6%,P＜0.05)or with IL-23 plus IgG in CD patients (50.2%±8.7%)and UC patients(51.5%±7.1%,P＜0.01).ConclusionIL-23 plays an important role in induction of NK cell activation and cytotoxicity in IBD patients,and may be involved in development of IBD.

Anticancer effect of mouse marrow-derived dendritic cells transfected by pcDNA3-hCEA / 中国病理生理杂志

AIM: To prepare the vaccine of DCs(pcDNA3-hCEA) and observ the immunity effect of the DCs(pcDNA3-hCEA) inoculating on CT26(hCEA +) loaded in BALB/c mice. METHODS: DCs were generated from bone marrow in the presence of rmGM-CSF and rmIL-4. A new recombinant plasmid, pcDNA3-hCEA, reformed with inserting a 2.4 kb human CEA cDNA into pcDNA3. DC vaccine was prepared by transfection with pcDNA3-hCEA using lipofectamine. CEA mRNA expressed in DCs(pcDNA3-hCEA) was confirmed by RT-PCR, CEA expression level was detected with RIA method, and CEA specific CTL was induced in vitro . After vaccination of DCs(pcDNA3-hCEA), the survival time of the BALB/c mice challenged with critical loading CT26(hCEA +) was observed. RESULTS: G418 test showed that about 14% DCs were transfected with pcDNA3-hCEA. And CEA mRNA and protein could be detected respectively by RT-PCR and RIA in the genetically modified DCs. Furthermore, the DCs coud be targeted by specific CTL, the survival time of the mice challenged with CT26(hCEA +) was prolonged 1-4 weeks. CONCLUSION: These results demonstrate that specific antitumor immune responses could be induced efficiently by vaccination of DCs(pcDNA3-hCEA), which is transfected eukaryotic expression vector encoding tumor antigen gene.