Factors influencing COVID-19 vaccination intention among parents of children aged 5-11 years in South Korea: a cross-sectional study

Purpose@#The purpose of this study was to identify factors affecting parents' intentions to have their children aged 5-11 years vaccinated against coronavirus disease 2019 (COVID-19). @*Methods@#The participants of the study were 298 parents with children aged 5-11 years in South Korea. Data collection took place from October 20 to October 26, 2022 and used an online survey (Google Forms). Data were analyzed using descriptive statistics, the t test, analysis of variance, the Scheffé test, Pearson correlation coefficients, and hierarchical regression in IBM SPSS version 26.0. @*Results@#The factors influencing participants' vaccination intentions for their children aged 5-11 years were cognitive behavioral control (β=.40, p<.001), attitudes (β=.37, p<.001), subjective norms (β=.20, p<.001), and awareness of whether their child could receive the COVID-19 vaccine (β=.07, p=.016). The explanatory power of the regression equation was 89%. @*Conclusion@#Parents' intentions to vaccinate their children against COVID-19 are influenced by their attitudes, subjective norms, and perceived behavioral control toward vaccines. Since parents are concerned about vaccine side effects, it is important to establish a trusted line of communication to keep them informed about vaccinations.

Effect of sonicates of Treponema denticola on osteoblast differentiation / 대한치주과학회지

No abstract available.

The effect of safflower seed fraction extract on periodontal ligament fibroblast and MC3T3-E1 cell in vitro / 대한치주과학회지

Recently, use of natural medicine is getting more attention, and some of them are believed to be effective in the treatment of periodontitis. Among them, the seeds of safflower(Carthamus tinctorius L.) have been proven to be effective through its use in bone diseases such as fracture and osteoporosis. During the last few years, studies using the seeds of safflower grown in Korea have been active, and it has been reported that safflower seed extract increase the proliferation and the alkaline phosphatase(ALP) activity of human periodontal ligament fibroblast(hPDLF), osteoblast, and that they promote the mineralization process. In animal studies, when safflower seed extract were administered orally new bone formation was promoted. Recently, in an effort to find out the most effective osteogenic components, among many components of the safflower seed, various safflower seed fraction extracts were obtained by multistep extraction of the safflower components using various solvents. Among these, saf-M-W fraction extracted by methanol and water was most effective in increasing osteogenic potential of osteoblasts. In this study, the effect of safflower seed fraction extract, saf-M-W, on the growth and differentiation of hPDLF and MC3T3-E1 cell was investigated. The toxicity of saf-M-W on both cells was measured using MTT(3- (4,5-dimethylthiazol-2-yl)-2,5- diphenyl tetrazolium bromide) test, and ALP activity was measured using the colorimetric assay of hPDLF. In addition, in MC3T3-E1 cells, the expression of ALP, bone sialoprotein(BSP) mRNA was observed using Northern blot, and the mineralized nodule formation was observed using von Kossa stain and phase-contrast microscope. 1. In concentrations below 10microgram/ml, saf-M-W didn't show any toxicity on hPDLF and MC3T3-E1 cell. 2. The change in saf-M-W concentration had no effect on the ALP activity of hPDLF. 3. In MC3T3-E1 cells, mRNA expressions of ALP and BSP were greater in the experimental group treated with 10microgram/ml concentration of saf-M-W compared with the control group. 4. In MC3T3-E1 cells, abundance of mineralized nodules were formed in the experimental group treated with 10microgram/ml concentration of saf-M-W, while no mineralized nodule was formed in the control group. These results suggest that safflower seed fraction extract, saf-M-W, didn't show any toxicity on hPDLF and MC3T3-E1 cell at concentrations below 10microgram/ml and effectively enhanced the differentiation and osteogenic potential of MC3T3-E1 cell.

The Effect of Cyclosporin A on Osteoblast in vitro / 대한치주과학회지

Cyclosporin A(CsA) is an immunosuppressive agent widely used for preventing graft rejecting response in organ transplantation. The basic properties of CsA to osteoblast has not been well known yet. A better understanding of the mechanisms of CsA function on bone could provide valuable information regarding basic properties of bone remodeling, pharmacotherapeutic intervention in metabolic bone disease, and the consequences of immunosuppression in bone physiology. The purpose of this study was to investigate the effect of CsA on osteoblast by evaluating parameters of proliferation, collagen synthetic activity, alkaline phosphatase activity, and ALP mRNA expression in mouse calvarial cell. 1. CsA(3microgram/ml) treated mouse calvarial cell showed statistically significant increase in cell proliferation.(P<0.05) 2. CsA(1, 3microgram/ml) treated MC3T3 cell line showed statistically significant increase in cell proliferation. 3. The amount of collagen of CsA(3microgram/ml) treated mouse calvarial cell was decreased statistically significantly. 4. Alkaline phosphatase activity was increased statistically significantly in CsA treated group(1microgram/ml). 5. mRNA expression of ALP was increased in CsA treated group These results suggest that CsA could affect bone remodeling by modulating proliferation & differentiation of osteoblast.

The Effects of Deer(Cervus nippon) Antler Extracts on Differentiation of MC3T3 Cells / 대한치주과학회지

Deer antler has been widely prescribed in Chinese and Korean pharmacology. Although there have been several reports concerning the effects of deer antler, such as anti-aging action, anti-inflammatory activity, antifungal action and regulatory activity of the level of glucose, the effect on bone has not determined yet. The purpose of this study was to examine the effect of deer antler on osteoblast differentiation. Hexane extract(CNH) and chloroform extract(CN-C) were acquired from deer antler(Cervus nippon) and MC3T3-E1 preosteoblasts were cultured in the presence or absence of each extract. Osteoblast differentiation was estimated with the formation of mineralized nodules and the mRNA expression of alkaline phosphatase(ALP), osteocalcin(OC) and bone sialoprotein(BSP) which are markers of osteoblast differentiation. Non-treated group did not show mineralized nodule. CN-C or CN-H-treated group showed minerlaized nodules in 16 days. In northern blot analysis, CN-C or CN-H-treated group showed the elevated expression of ALP, BSP and OC in 16 days. These results suggest the possibility to develop deer antler as a bone regenerative agent in periodontal therapy by showing the stimulating activity of deer antler on differentiation of osteoblast.