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Objective To evaluate the role of heme oxygenase-1 (HO-1) in sevoflurane-induced reduction of airway injury in asthmatic mice.Methods Forty SPF female C57BL/6 mice,aged 6-8 weeks,weighing 20-25 g,were divided into 4 groups (n=10 each) using a random number table:control group (group C),asthma group (group A),asthma plus sevoflurane group (group A+S) and asthma plus sevoflurane plus zinc protoporphyrin group (group A+S+Z).The mice were sensitized and repeatedly challenged with ovalbumin to establish the asthma model.In group A+S,3％ sevoflurane was inhaled for 1 h after every challenge.In group A+S+Z,zinc protoporphyrin 10 mg/kg was intraperitoneally injected after every challenge and before sevoflurane inhalation.The airway responsiveness were assessed at 24 h af=ter the last challenge.The bronchoalvelor lavage fluid (BALF) was collected for determination of cell type and count and concentrations of interleukin-13 (IL-13) and IL-33 (by enzyme-linked immunosorbent assay).The right lung tissues were obtained for measurement of the superoxide dismutase (SOD) activity,contents of malondialdehyde (MDA) and glutathione (GSH) and expression of HO-1 (by Western blot).Lung tissues were stained with haematoxylin and eosin and inflammatory cell infiltration was assessed and scored.Results Compared with group C,the airway responsiveness,inflammatory cell count and concentrations of IL-13 and IL-33 in BALF,and MDA content and infiltration score in lung tissues were significantly increased,the SOD activity and GSH content were decreased,and the expression of HO-1 was upregulated in group A (P＜0.05).Compared with group A,the airway responsiveness,inflammatory cell count and concentrations of IL-13 and IL-33 in BALF,and MDA content and infiltration score in lung tissues were significantly decreased,the SOD activity and GSH content were increased,and the expression of HO-1 was up-regulated in group A+S (P＜0.05),and the expression of HO-1 was down-regulated (P＜ 0.05),and no significant change was found in the other parameters mentioned above in group A+S+Z (P＞ 0.05).Compared with group A+S,the airway responsiveness,inflammatory cell count and concentrations of IL-13 and IL-33 in BALF,and MDA content and infiltration score in lung tissues were significantly increased,the SOD activity and GSH content were decreased,and the expression of HO-1 was down-regulated in group A+S+Z (P＜0.05).Conclusion HO-1 is involved in the pathophysiological mechanism by which sevoflurane attenuates airway injury in asthmatic mice.
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Objective To explore the therapeutic effect of Cefoselis sulfate and Cefepime on the stroke-associated pneumonia in elderly patients.Methods 96 cases of stroke-associated pneumonia with positive pathogen were divided into two groups treated with Cefoselis versus Cefepime respectively.Body temperature,leukocyte count,arterial partial oxygen pressure,the serum level of C-reactive protein (CRP) and bacterial clearance rate were observed before and after the treatment.The cefoselis sulfate group (n =48) or the cefepime group (n =48) was treated with 2.0 g Cefoselis sulfate or 2.0 g Cefepime every 12 hours for 7 to 10 days.Results In post-treatment versus pretreatment,both two groups showed that clinical symptoms and inflammatory indicators were improved,and the patient's body temperature,white blood cell count and neutrophil cell count,hs-CRP and arterial partial oxygen pressure were significantly decreased (all P＜0.05).Arterial partial oxygen pressure was better in post-versus pre-treatment (P＜0.05).The total effective rate was 87.5％ in Cefoselis sulfate group and 83.3％ in cefepime group (P ＞ 0.05).The bacterial clearance rate was higher (89.6％) in Cefoselis sulfate groups than in cefepime groups (67.9％) (P＜0.05).In posttreatments of Cefoselis sulfate groups versus Cefepime groups,white blood cell count and neutrophil cell count,hs-CRP and arterial partial oxygen pressure showed no statistically significant differences (all P ＞ 0.05).Conclusions Compared with Cefepime,Cefoselis has the similar anti-infection efficacy in the treatment of stroke-associated pneumonia.While,the bacterial clearance rate and the total effective rate are better in cefoselis group than in Cefepime group.