Determination of Cyclovirobuxium D in Huangyangning Pharmaceutical Preparations by UPLC-MS/MS / 中国药师

Objective:To establish a method for the determination of cyclovirobuxium D in Huangyangning pharmaceutical prepa-rations by UPLC-MS/MS. Methods:UPLC-MS/MS was conducted. The column was Waters ACQUITY UPLC BEH C18column(100 mm×2.1 mm,1.7 μm), the mobile phase consisted of acetonitrile and 0.01 mol· L-1ammonium formate solution (containing 0.1% formic acid) with gradient elution at a flow rate of 0.35 ml· min-1. The column temperature was 30℃ and the injection vol-ume was 5 μl. The ionization mode was electrospray ionization (ESI) with a positive mode. Cyclovirobuxium D was analyzed with a multiple-reaction monitoring mode (MRM). Results: The linear range of cyclovirobuxium D was 15.58-1558.00 ng· ml-1(r =0.999 5). The average recovery of cyclovirobuxium D was 97.7%(RSD=3.8%,n=6). Conclusion:The method is rapid,simple and accurate with good reproducibility. It is applicable in the determination of cyclovirobuxium D in Huangyangning pharmaceutical preparations to be beneficial to the quality control of Huangyangning pharmaceutical preparations.

Quantitative assessment of left ventricular long axis systolic function in patients with abdominal obesity using tissue mitral annular displacement / 中华超声影像学杂志

ObjectiveTo evaluate the value of tissue mitral annular displacement (TMAD) in the assessment of left ventricular long axis systolic function and the relationship of obese degree with left ventricular long axis systolic function in patients with abdominal obesity.MethodsThirty-eight abdominal obesity cases and thirty-four healthy cases were investigated using echocardiography.The images of apical four-chamber view and apical two-chamber view were obtained,systolic mitral annular displacement (MADs),mid-point of mitral annular displacement(MAD-midpt),mid-point of mitral annular normalized displacement(MAND-midpt) and mid-point of mitral annular biplanar normalized displacement(MABNDmidpt) were measured by the technique of TMAD.Their characteristics between patients with abdominal obesity and healthy group were compared,and the relationship of waist-hip ratio(WHR) and related indexes of MAD were analyzed.ResultsIn abdominal obesity group,the MADs at the four site were significantly decreased compared with control group (P＜0.001,respectively),the MAD-midpt and MAND-midpt of apical four-chamber view and apical two-chamber view were significantly decreased compared with control group (P＜0.001,respectively).The WHR related with MABND-midpt independently by the analysis of partial correlation(r=-0.697,P=0.000).ConclusionsThe left ventricular long axis systolic function were damaged in patients with abdominal obesity.The technique of TMAD could quantitatively assess the left ventricular long axis systolic function in patients with abdominal obesity.