The role of transcription factors in differentiation and development of the olfactory epithelium stem cells / 中华耳鼻咽喉头颈外科杂志

As a sensor of the olfactory system, olfactory epithelium plays an important role in the olfactory system. In addition, olfactory epithelium is the only neuroepithelial epithelium in mammals that can maintain its self-renewal all along. There are of great significance in researching regenerating and repairing neural tissues, transplanting and treatment neural stem cells as well as the occurrence of olfactory disorders and intervention. This review will describe the characteristics of olfactory epithelial stem cells, and mainly summerize the function and significance of each transcription factor in the process of olfactory epithelial stem cell development and differentiation, in order to provide new ideas for the study of olfactory epithelial stem cells.

The cultivation and migration in vitro of olfactory ensheathing cells from human olfactory mucosa / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To investigate the bionomics of the olfactory ensheathing cells (OECs) of human olfactory mucosa.@*METHOD@#To separate and cultivate the OECs of human and rat olfactory mucosa. To observe the cell growth, cell grouping and cell migration in vitro of the two types of OECs.@*RESULT@#Successfully separated and cultivated the OECs of human and rat olfactory mucosa. OECs of the human and rat olfactory mucosa had the similar cell growth, cell grouping and cell migration ability in vitro.@*CONCLUSION@#OECs of the human and rat olfactory mucosa have the similar bionomics in vitro, as a result, OECs of the human olfactory mucosa could be a reliable source of cell transplant for nerve injury.

OSAHS patient gas up-take cross-sectional area nasopharynx sound reflection examination and significance / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To explore a simple and accurate method for localization of upper airway obstruction in patients with obstructive sleep apnea-hypopnea syndrome (OSAHS) and provide instructions for surgical treatment.@*METHOD@#Fifty OSAHS patients confirmed by PSG underwent acoustic rhinometric and pharyngometric assessment by Eccovision. The parameters were recorded, including nasal minimal cross-sectional area (NMCA), distance of MCA from the nostril (DCAN), minimum cross-sectional area at the nasal valve(MCA), nasal resistance (NR) and nasal volume from 0 to 6 cm from the nostril (NCV), as well as pharyngeal cross-sectional area (CSA) and volume from 4.8 to 15.0 cm. The sensitivity and specificity of acoustic rhinometry and pharyngometry on localization of airway obstruction was determined by a comprehensive imaging and endoscopic study.@*RESULT@#In 50 cases with severe OSAHS, NMCA, DCAN, MCA, NCV, NR were (0.61 +/- 0.35) cm2, (2.06 +/- 0.12) cm, (0.87 +/- 0.12) cm2, (9.24 +/- 2.31)cm3 and (0.51 +/- 0.32)kPa/(L x min), respectively. Pharyngeal CSA and volume were statistically significantly lower than that in control group (P < 0.01). The value of DCAN was (2.06 +/- 0.12) cm, (9.50 +/- 4.08) cm, (13.10 +/- 2.52) cm in type I II, III patient, respectively. Compared with the control group, the difference was statistically significant.@*CONCLUSION@#Acoustic rhinometry and pharyngometry is a simple and safe method in localization of airway obstruction in patients with OSAHS.

Expression of complement regulatory proteins C_1-INH and CD46 in nasal mucosa of allergic rhinitis rats / 第二军医大学学报

Objective:To determine the expression of complement regulatory proteins(CRPs) C_1-INH,MCP(CD46) in the nasal mucosa of allergic rhinitis rats and to study the role of CRPs in the pathogenesis of allergic rhinitis.Methods: Nine healthy SD rats were sensitized and intranasally challenged with ovalbumin and Al(OH)_3(as supplement) to establish allergic rhinitis models and another 9 SD rats treated with saline were taken as control.The nasal mucosa in respiratory area of both groups was obtained.Then Western blotting was performed to investigate the expression levels of C_1-INH and CD46.Results: Western blotting showed that both C_1-INH and CD46 had been detected in rat nasal mucosa.Expression level of CD46 in the nasal mucosa of allergic rhinitis was significantly higher than that in control rats(P