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Objective To investigate the effect of programed training in the treatment of patients with chronic schizophrenia.Methods 88 patients with chronic schizophrenia were randomly divided into families involved in the training group and control group 44 cases,the training group for eight weeks of programmed training,and then all were followed up for 44 weeks.Positive and negative syndrome scale (PANSS),insight and treatment attitude questionnaire(ITAQ),Morningside Rehabilitation Status Scale(MRSS) were selected to assess.Results Total score of PANSS,negative symptoms and general psychopathology in The training group sub-section 20,32,44 weekend were cignificartly lower than in the control group(t =2.42 ～ 11.19,all P ＜0.01) ;positive symptoms points in 44 weekend the difference was significant(t =3.19,P ＜ 0.01) ; training group ITAQ score the weekend of the first 20,32,44 were significantly higher(t =3.13 ～ 8.32,all P ＜ 0.01) ;training group MRSS assessment dependent activities,the lack of capacity,the average social skills and the current symptoms and abnormal behavior in the 20 weeks were significantly lower than the control group.(t =1.67 ～4.31 ;2.37 ＞ t ＞ 1.664,P ＜ 0.05 ;t ＞ 2.374,P ＜ 0.01) ;relapse rate after 1 year of follow-up two groups by the x2 test (x2 =0.87 ;P ＜ 0.05) have significant differences.Conclusion The programmable training can significantly improve the effect of rehabilitation of patients with chronic schizophrenia,reduce the recurrence rate of chronic schizophrenia.
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To observe whether Naomai Yihao (NM) Capsule, a compound traditional Chinese herbal medicine for regulating the "sea of blood in brain", and bone marrow stromal stem cell (BMSC) transplantation could improve angiogenesis in focal cerebral ischemia in rats.
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Fas-associated death domain (FADD) is a signal connection protein in Fas/FasL apoptotic path which might play a key role on apoptosis by transferring apoptotic signal. To reveal the intracellular signal transduction molecules involved in the procedure of follicular development in bovine ovary, we cloned FADD gene in bovine ovary tissue with RT-PCR, deleted the termination codon in its cDNA and directionally cloned the amplified FADD gene into eukaryotic expression vector pAcGFP-N1 including AcGFP, successfully constructed the fusion protein recombinant plasmid. After identifying by restrictive enzyme Bgl II/EcoR I and sequencing, transfected pAcGFP-bFADD into CHO-K1 cell mediated by Lipofectamine 2000, observed the expression of AcGFP and detected the transcription and expression of FADD by RT-PCR and Western blotting. The results showed that the cattle FADD was successfully cloned, the pAcGFP-bFADD fusion protein recombinant plasmid was successfully constructed by introducing Bgl II, EcoR I cloning site at two ends of FADD open reading frame and inserting a Kozak sequence before start codon. AcGFP expression was detected as early as 24 h after transfection. The percentage of AcGFP positive cells reached about 65% after 24 h. A 654 bp transcription was amplified by RT-PCR, and 51.4 kD target protein was detected by Western blotting. Construction of pAcGFP-bFADD recombinant plasmid should be helpful for further understanding the mechanism of regulation of FADD on bovine oocytes formation and development.
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Animais , Bovinos , Cricetinae , Feminino , Sequência de Bases , Células CHO , Clonagem Molecular , Cricetulus , DNA Complementar , Genética , Proteína de Domínio de Morte Associada a Fas , Genética , Vetores Genéticos , Genética , Proteínas de Fluorescência Verde , Genética , Dados de Sequência Molecular , Oócitos , Biologia Celular , Fases de Leitura Aberta , Ovário , Metabolismo , Proteínas Recombinantes de Fusão , Genética , TransfecçãoRESUMO
Recent animal experiments demonstrated that neurogenesis mad angiogenesis involxed in mechanisms of stroke recovery.Transplanted stem cells can improve the neurological function after stroke.Vascular endothelial growth factor can also improve the neurological function after stroke via enhenceing angiogenesis.This article reviews the advances in combined treatment of stem cells and vascular endothelial growth factor in ischemic brain injury.