RESUMO
Objective:To establish a quality control method for Jingdu Niancian Milian Chuanbei Pipagao by quantitative analysis of multi-components by single marker ( QAMS) . Methods:GC was used with benzaldehyde as the internal standard,and the relative calibration factor of benzaldehyde and menthol was establish by GC and used for the content calculation of menthol by QAMS. Mean-while,the results of QAMS and those of the external standard method were compared. The effects of different instruments and chromato-graphic columns were studied to evaluate the accuracy and scientificity of QAMS applied in compound preparations. Results:No signif-icant differences in the quantitative results of benzaldehyde and menthol were found between the external standard method and QAMS. Conclusion:It is feasible and suitable to evaluate the quality of benzaldehyde and menthol in Jingdu Niancian Milian Chuanbei Pipagao by QAMS.
RESUMO
Objective:To establish a quality control method for Jingdu Niancian Milian Chuanbei Pipagao by quantitative analysis of multi-components by single marker ( QAMS) . Methods:GC was used with benzaldehyde as the internal standard,and the relative calibration factor of benzaldehyde and menthol was establish by GC and used for the content calculation of menthol by QAMS. Mean-while,the results of QAMS and those of the external standard method were compared. The effects of different instruments and chromato-graphic columns were studied to evaluate the accuracy and scientificity of QAMS applied in compound preparations. Results:No signif-icant differences in the quantitative results of benzaldehyde and menthol were found between the external standard method and QAMS. Conclusion:It is feasible and suitable to evaluate the quality of benzaldehyde and menthol in Jingdu Niancian Milian Chuanbei Pipagao by QAMS.
RESUMO
Objective:To establish the quality standard for Sinapis Semen( stir-baked) formula granule. Methods:TLC was used to identify Sinapis Semen; an HPLC method was applied in the content determination of Sinapine thiocyanate in Sinapis Semen ( stir-baked) formula granule with Hibar Purospher STAR C18 (250 mm × 4. 6 mm,5 μm ) column and with the mobile phase consisting of acetonitrile-0. 08 mol·L-1 potassium dihydrogen phosphate (10 ∶90) at the flow rate of 1. 0 ml·min-1 ,the column temperature was 35℃ and the detection wavelength was set at 326 nm. Results:The characteristic spots of Sinapis Semen( stir-baked) formula granule were clearly detected by the established TLC chromatography. Sinapine thiocyanate had a good linear relationship within the concentra-tion range of 0. 012 2-3. 912 3μg(r=0. 999 9). The average recovery was 100. 4% (RSD=0. 6%, n=6). The water content, dis-solution and particle size of Sinapis Semen formula granule all met the related requirements. Conclusion:The methods are simple and accurate with good reproducibility,which can be used to control the quality of Sinapis Semen( stir-baked) formula granule.