Chromosomal Abnormalities in Embryos

From patients with a poor prognosis of pregnancy, 1620 embryos generated in vitro and chromosomal analysis was performed on these embryos. The result was yielded in 1596 embryos, out of them 536(34%) were euploid and 1060(66%) carried chromosomal abnormalities. In addition, 92% of embryos with multinucleated cells were diagnosed mosaics whereas the 86% of chromosomal abnormalities were associated to the presence of cytoplasmic concentration. For the derivation of the normal embryonic stem cell (ESC)lines and developmental modelling aneuploid embryos have been used. Genetic diagnosis at the cleavage or blastocyst stage could be partly abnormal because during the preimplantation diploid- aneuploid mosaic embryos was most frequently observed. From a single cell of a particular embryo the chromosomal status of that embryo can be determined, thus the prevalence of mosaicism. Detection of aneuploidy in single cells have been developed recently. After conducting research methods, it was confirmed that aneuploidy is a common feature of human oocytes and preimplantation embryos. The detection of segmental aneuploidy is currently considered problematic for embryo diagnosis and patient counselling, so the data are of great relevance for preimplantation genetic testing. The first major milestone in early mammalian embryogenesis was the formation of a totipotence blastocyst which is capable of implantation. The whole chromosomal abnormalities, or aneuploidy, determines whether the human embryos will arrest or reach the blastocyst stage. Certain embryos can still form blastocyst depending on the type of chromosomal abnormalities and that can be morphologically indistinguishable from chromosomally normal embryos.

Assessment of the customized birth weight formula in a low risk Indian population

Background: Birth weight is probably the single most important factor that affects neonatal mortality, infant and childhood morbidity in both developed and developing countries. Objective of the study was to formulate a model for prediction of fetal weight at term based on individualized fetal growth parameters.Methods: 131 participant low risk gravidas were enrolled into the study. The participants underwent an ultrasound 7 days prior to delivery. All fetal variables of growth of a random 100 participants were incorporated in an equation derived using multiple regressions to predict birth weight at term. The new equation was then prospectively applied to another 31 pregnant women for validation. The diagnostic performance of the new regression formula was then compared to the Hadlock formula.Results: The customised birth weight formula predicted a higher accuracy with MPE±SD of 0.790±9. compared to the Hadlock formula with MPE±SD - 4.42±8.73. The new formula also explained a greater variance in birth weight of 56% compared to the Hadlock formula of 49%.Conclusions: The new model based on individualized fetal growth parameters recognizes the capacity to modulate an accurate final birth weight, thus emphasizing the need for customized population specific birth weight formulas.

Morphometric Study Of Nutrient Foramen Of The Dried Human Tibiae

Background: Nutrient foramen is an opening over which the nutrient artery enters and supplies the shaft of thelong bones. The nutrient foramen usually lies near the soleal line and transmits a branch of the posterior tibialartery. The posterior tibial artery is a branch from the popliteal artery. The nutrient vessel may also arise at thelevel of the popliteal bifurcation or as a branch from the anterior tibial artery.Methods: The present study was conducted on 200 dry human tibia bones of unknown sex and age. The tibiabones were observed for nutrient foramen macroscopically.Results: In our study the right sided 7 tibia bones and 6 left sided tibia bones have double nutrient foramen. Theposition of the nutrient foramen in the upper 1/3rd is observed in 77.47% tibia and in the middle 1/3rd in 17.84%of the tibia.The direction of the nutrient foramen is downwards in all the tibia bonesConclusions: The tibia is the most commonly fractured long bone and contributes significantly to the fracturecare worldwide. Fracture of the tibia through the nutrient canal disrupts the blood flow in the nutrient artery,thus contributing delayed union and non-union of the bone. Knowledge of the blood supply and location ofnutrient foramen is important in the treatment and planning of surgery in fractures.

Use of Learning Platforms for Quality Improvement

Ensuring quality in healthcare today has become extremely essential to ensure adequate utilization of healthcare services andimproved outcomes. In addition to essential infrastructure in terms of safe and adequate space, knowledgeable and skilled healthworkers, and essential equipment and supply, the healthcare teams and administrators must also acquire knowledge and skills relatedto quality improvement (QI) methodologies. This review describes the role of learning platforms in teaching QI skills to the busyhealthcare teams. Through Review of the published literature, we discuss challenges of learning and applying new skills of QI in day-to-day work by healthcare teams, and how learning platforms can assist in capacity building. There is a significant body of literature onthe role of web-based teaching technology and learning platforms in medical education. Using modern communication technology,learning platforms can be established to bring together the healthcare teams, with QI experts to collaboratively learn, execute andshare their experiences in improving quality of care in their own healthcare settings.

Prevalence & risk factors for hepatitis C virus among pregnant women.

BACKGROUND & OBJECTIVE: Information on hepatitis C virus (HCV) infection in pregnant women in India is scanty. This study was carried out to investigate the prevalence of HCV within an obstetric population in north India and to identify the various risk factors for the viral infection. METHODS: A total of 8130 pregnant women from antenatal clinic were subjected to anti-HCV testing by third generation ELISA. Anti-HCV positive seropositive women were further tested for HCVRNA, hepatitis B and HIV. The women were evaluated for the presence of following known risk factors for HCV infection. RESULTS: Eighty four (1.03%) pregnant females had HCV antibodies. Of these, 46 (54.8%) were positive for HCV-RNA, 4(4.8%) tested positive for HBsAg, while none tested positive for HIV. The mean age and parity of the anti-HCV antibody positive women was 24.36+/-3.6 yr and 0.9+/-0.8, while that of the anti-HCV antibody negative women was 24.13+/-3.6 yr and 0.8+/-0.8 respectively. Of the 84 anti-HCV positive women, 52 (61.9 %) did not have any identifiable risk factors. The risk factors variables did not have significant association with HCV positive status. INTERPRETATION & CONCLUSION: Prevalence of hepatitis C in pregnant women was 1.03 per cent. None of the known risk factors was found to be significantly associated with the HCV infection. Hence case identification and consequent management pose a particular problem and routine screening is not a viable option in our resource- poor setting.