RESUMO
Background and Objective: MiRNAs are a systematic class of small non-coding RNAs with impending role; tumor biomarkers. Our objective was to identify the level of expression of Mir-195 in patients with brea cancer along with its correlation with clinicopathologic features
Methods: A total of 209 females in which 139 historically diagnosed breast cancer [BC] cases and] healthy controls matched for age, their relative clinical and histopathological findings were recorded fro their laboratory reports and hospital record of the patients. Plasma was used for extraction of total l" and cDNA was prepared by using both miR-195 stem loop RT primers and gene specific antisense prime while U6 IT was used as control. Quantitative real-time PCR [qRT-PCR] for miR-195 expression status w< performed and amplification [down regulation] was recorded
Results: Of 139 samples the expression of miR-195 was down regulated in 72.6% cases and the remains 27.3% cases behaved same as 70 healthy or normal controls. Significant correlation of low miR-15 expression with higher differentiation grade, lymph node metastasis and clinical stage was found
Conclusion: Significant correlation between miR-195 expression and some Clinicopathological feature were recognized. MiR-195 could be used as potential non-invasive, molecular biomarker for early detectio of breast cancer