RESUMO
Leishmaniasis is a multisystem disease, and have a broad spectrum ranging from skin lesions to systemic disease. Therefore diagnosis must be supported with laboratory results. We analyzed 153 smears, aspiration, blood and bone narrow samples collected from patients suspected with cutaneous and visceral leishmaniasis. The specificity and sensitivity of the four methods (culture, smear, miniexon-PCR-RFLP and ITS1-PCR-RFLP) were detected and Leishmania species were determined. The ITS1-PCR-RFLP method was found that the highest sensitivity and specifity. L. infantum and L. tropica were identified by molecular methods from samples. As a result, ITS-1-PCR has a high sensitivity and specificity and easily applicable method. However, it requires the miniexon-PCR or ITS1 sequencing the discrimination of the L. donovani complex. L. infantum is a agent both visceral and cutaneous leishmaniasis in our region.
RESUMO
The prevalence of IgG antibodies to C. trachomatis was determined in 185 infertile women who underwent laparoscopy, and 110 pregnant women with no known infertility problems. In addition, chlamydial antigens were evaluated by EIA in cervical samples taken from all subjects. Subjects with tubal infertility had the highest prevalence of chlamydial antigen and antibody (P less than 0.01). While the percentage of subjects with antigen and antibody positive was 11.6 per cent, those with antigen negative and antibody positive averaged 55.8 per cent among women with tubal related infertility. The results of our study provide additional support to the concept that infertility of tubal etiology often is a sequela of a previous chlamydial infection.