Requirements of Opsono-phagocytosis of staphylococci

Opsonophagocytosis of both Staph. aureus and coagulase negative staphylococci was studied in 46 strains from different sources of infection. Opsonophagocytosis was found to be mediated by the antibody and complement [in normal human serum] but could be also mediated by antibody alone in absence of the complement [in heated serum]. Also, it could be mediated by the complement alone in the absence of the antibody [in absorbed serum]. Coagulase negative staphylococci was more susceptible to opsonophagocytosis than Staph. aureus strains. No difference in opsonophagocytosis was found according to difference of sources of isolation