RESUMO
Background and Objective: Iron oxide nanoparticles have wide applications such as MRI contrast agent and drug delivery. Nevertheless, their effects on human health have not been fully investigated yet. After cellulose, chitin is one of the most abundant organic materials in nature which is widely used in food industry, cosmetics, agriculture, medicine and the environment. This study was done to evaluate the effect of iron oxide nanoparticles coated with chitosan on renal functional indeces in rat
Methods: In this experimental study, 60 adult female Wistar rats were allocated into 10 equal groups. Concentrations of 50, 100 and 150 mg/kg/bw from chitosan, iron oxide nanoparticles and chitosan coated nanoparticles were intraperitoneally injected into 9 groups and animals in control group were received normal saline. Blood samples were collected directly from the rat heart in the days 15 and 30 post after injection and renal functional indeces including urea, creatinine, uric acid, sodium, potassium and total protein were measured
Results: There were no significant differences in the level of urea, creatinine, uric acid, sodium, potassium and total protein in the groups whom received chitosan-coated iron oxide nanoparticles compared to control. There was no mortality during the study time
Conclusion: Short-term using of iron oxide nanoparticles coated with chitosan does not create any toxicity in the rat kidney
RESUMO
Background: one of the complications of diabetes mellitus is "proteins glycosylation in the body" causing the change in nature, structure and biochemical activity of them. Decreasing or inhibiting this reaction causes to improve diabetic symptoms. The use of antioxidant-rich herbs in this case, seems to be effective
Objective: the purpose of this study is to investigate the albumin and hemoglobin glycosylation reaction in the presence of various concentrations of Anethum graveolens and Rosa damascena Mill hydroalcoholic extracts
Methods: 1] plants collection, 2] extraction by maceration method, 3] evaluating the total antioxidant capacity and phenole amount respectively by DPPH and folin-Ciocalteu method, 4] specializing and measuring the amount of hemoglobin from human blood, 5] optimizing the stabilization, 6] investigating the glycosylation amounts, both in the presence and absence of extracts
Results: according to results obtained, the phenol amount and the total antioxidant capacity of Anethum graveolens hydroalcoholic extract is significantly more than Roses's [P<0.05]. The highest amount of hemoglobin glycosylation per time unit, has been at a concentration of 10 mgr glucose for 72 hours of incubation and about albumin was 30 mgr within 72 hours. For both extracts in concentration 0.1microg / ml of both proteins, the Anethum graveolens hydroalcoholic extract functions better compared to Rosa damascena Mill [P <0.05]
Conclusion: the two Anethum graveolens and Rosa hydroalcoholic extracts on average cause to reduce albumin and hemoglobin glycosylation. But more studies are needed to prove it