Carditis in experimental trichinosis

Eighty white rats were infected with 1000 larvae of Trichinella spiralis/rat. Serum samples and the hearts of infected animals were taken weekly for 20 weeks after infection. Tissues from the heart were stained with hematoxylin and eosin and Mallory stains to demonstrate histopathological changes and with peroxidase anti- peroxidase for the detection of specific antigen deposition. Early in the disease, myocardial changes were detected. Later on, pericarditis and interstitial myocarditis were found followed by patchy myocardial necrosis and fibrosis. Antigen deposition was demonstrated mainly on sarcolemmal sheath of myocardial muscle fibers and in endothelial cell lining of blood vessels. No larvae were found in heart sections throughout the study. The pathological changes were mainly attributed to the antigen deposition and subsequent immune reaction

Expression of T-cells and their subsets in different types of non hodgkin lymphoma

Eighteen cases diagnosed histopathologically as malignant lymphomas with different subtypes were subjected to immunological study to express their content of T-cells and their subsets. They were stained by the Avidin-Biotin complex method with the use of monoclonal antibodies against helper/inducer and suppressor/cytotoxic subsets [namely T4 and T8 monoclonal antibodies]. The findings demonstrated the presence of T-cells with its subsets in nearly all cases of malignant lymphomas but with different proportions. A few number of helper T4+ cells were demonstrated intrafollicular in cases of follicular lymphoma, while a larger number was seen in diffuse lymphomas mainly in lymphoblastic, large cell and small cleaved cell subtypes with predominence of T4+ helper over T8+ suppressor subject. Meanwhile, this was reversed in diffuse mixed subset where T8+ suppressor subset dominated