Immune response of chicken infected with Quaranfil virus

The immune response of chicken infected with Quaranfil [QRFV] virus was investigated. The dose response curve of whole blood culture and spleen lymphocyte cultures were detected by non specific stimulation with phytohaemagglutinin [PHA] at different dilutions. The optimium stimulation was [1:225] with 0.5x10[6] cells in whole blood culture, also [1:225] in spleen lymphocyte culture. The delayed hypersensitivity skin test as a sequential reactivity post injection with specific antigen revealed moderate reaction against QRFV. The plaque forming cells [PFC] was reduced in case of QRFV if it is compared with the control group [sheep red blood cells]