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Indian J Exp Biol ; 2000 Nov; 38(11): 1143-6
Artigo em Inglês | IMSEAR | ID: sea-57153

RESUMO

Aeromonas sp. from Lamellidens marginalis produced L-asparaginase when grown at 37 degrees C. The optimum enzyme activity was at pH 9 when temperature was 45 degrees C. Half-life of partially purified enzyme at 50 degrees C and 55 degrees C was 35 and 20 min, respectively. Activation and deactivation energies of partially purified enzyme were 17.48 and 24.86 kcal mol-1 respectively. The enzyme exhibited a Km (L-asparagine) value of 4.9 x 10(-6) mol l-1 and a Vmax of 9.803 IU ml-1. Three metal ions inhibited the enzyme activity at 10-20 mumol l-1 concentrations. Catalytic activity was also inhibited by EDTA, iodoacetic acid, parachloromercuribenzoic acid and phenylmethylsulphonyl fluoride at 0.1 mumol l-1.


Assuntos
Aeromonas/enzimologia , Animais , Asparaginase/metabolismo , Bivalves/microbiologia , Temperatura Alta , Concentração de Íons de Hidrogênio , Metais/metabolismo
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