RESUMO
Blastocystis hominis is a common, yet neglected parasite with world-wide distribution. A cross-sectional study was conducted to determine the prevalence of Blastocystis hominis and its relation to the quality of drinking water supply among hospital food handlers in Menoufiya governorate. Total of [313] hospital food handlers were enrolled in this study. Data were collected via a predesigned questionnaire. Stool specimens were examined by simple direct smear, zinc sulphate concentration floatation methods, Ziehl-Neelsen and Safranin blue stains. All water samples were subjected to physical, chemical and bacteriological examination. Results revealed that the prevalence rate of B hominis was 5.7% [11.4% among symptomatic and 3.8% among asymptomatic ones]. Frequent attacks of diarrhea and abdominal discomfort were the most common complaints. It was more prevalent among young adults and assistant food handlers [P<0.05]. There was no significant difference between food handlers with or without B. hominis regarding quality of their drinking water supply. However, some physical, chemical and bacteriological parameters of water quality exceed the acceptable drinking water quality standard in both groups. In conclusion, Blastocystis hominis is not uncommon protozoal infection associated with gastrointestinal manifestations among hospital food handlers