Determination of β-lactoglobulin in Anti-HPV Biological Protein Dressing by Amino Acid Assay / 中国医疗器械杂志

OBJECTIVE@#To establish an amino acid assay for the determination of β-lactoglobulin in Anti-HPV biological protein dressing.@*METHODS@#Under acidic conditions, β-lactoglobulin is hydrolyzed into free amino acids, separated by cation exchange chromatography, and derivatived after ninhydrin column. The chromatogram at 570 nm is collected. The content of β-lactoglobulin in the sample is indirectly determined by measuring the lysine content obtained by hydrolysis.@*RESULTS@#β-lactoglobulin has a good linear relationship in the concentration range of 77.28~309.12 μg/mL (@*CONCLUSIONS@#The method is simple, specific, accurate and reproducible, which is suitable for the quantitative analysis of β-lactoglobulin in anti-HPV biological protein dressing.

Determination of the Related Substances in Sertaconazole Nitrate Vaginal Tablet by HPLC / 中国药房

OBJECTIVE:To establish a method for determination of the related substances in Sertaconazole nitrate vaginal tab-let. METHODS: HPLC was performed on the column of Hypersil BDS C18(150 mm×4.6 mm,5 μm)with mobile phase of 0.5%Ammomium acetate solution-acetonitrile-methanol(15∶42.5∶42.5,V/V/V) at a flow rate of 1.0 ml/min,detection wavelength was 220 nm,column temperature was 25℃,and the injection volume was 20μl. RESULTS:The related substances in Sertaconazole ni-trate vaginal tablet can be well separated;the liner range of sertaconazole nitrate was 12.24-28.56 μg/ml (r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 1%;recovery was 99.38%-99.80%(RSD=0.14%,n=9). CONCLU-SIONS:The method is accurate and reliable with high sensitivity and strong specificity,and can be used for the related substances in Sertaconazole nitrate vaginal tablet.

Determination of the Titer of Azithromycin by Turbidimetric Method / 中国药房

OBJECTIVE: To develop a method for the determination of the titer of azithromycin. METHODS: Turbidimetric method was adopted to determine azithromycin and which was compared with cup-plate method. RESULTS: The linear range of azithromycin was 0.4～2.0IU?mL-1(r=0.999 1), with its recovery rate at 99.70%～100.02%(RSD=0.22%～0.46%), the determination results of 2 kinds of methods was no significant difference. CONCLUSION: Turbidimetric method was proved to be more rapid and convenient than the cup-plate method.