RESUMO
According to one pot microwave assisted synthesis, the versatile precursor 2- aminothiazole was prepared and utilized for the construction of new thiazole hybrids targeting MCF7 cell lines. 2‐amino thiazole was condensed with corresponding aldehydes to yield Schiff’sbase (2) intermediates followed by the diazo coupling reaction furnished the designed hybrids(3) contains azo-methine and diazo linkages in its structures. The newly synthesizedcompounds were confirmed on the basis of IR and H1NMR spectral analytical data. All thesynthesized compounds were evaluated for their in-vitro cytotoxicity activity against MCF-7celllines using MTT assay method. The obtained results revealed the more promising compoundsof the synthesised series, 3B and 3H with CTC50 value of 17.77±0.31μg/ml, 17.83±1.14 μg/ml.
RESUMO
Background: Dental caries is the destruction of enamel, dentin, or cementum of teeth due to bacterial activities, which if left untreated can cause considerable pain, discomfort, and treatment costs are very high. Of the oral bacteria, Streptococcus mutans is considered to be causative agent of dental caries in humans. Aim: This study aims at screening the antibacterial potential of available oral hygiene products against S. mutans, the primary etiological agent. Materials and Methods: A selective number of toothpaste and mouth rinse available in the nearby local market was subjected to the study. The experiments were designed in a way to determine which one of these products had the greatest anti-S.mutans activity. Antibiotic sensitivity tests against the pathogenic strains were also conducted. Results and Discussion: Pathogenic strains of S.mutans were isolated from clinical dental specimen and identified using MTCC standard strain No. 890. Of the 86.66% samples which showed positive for S.mutans growth, almost 96% conferred sensitivity to 0.08 mg/ml of penicillin. Among the toothpastes used, A showed the maximum inhibitory activity against S.mutans inhibiting its growth even at a very low concentration of 0.0156 g/ml. Similar results were seen in the case of the mouth rinses used for the study, which showed that N had the most effective activity against S.mutans even at 1:8 dilution. Conclusion: The oral hygiene products containing triclosan proved to be the most effective followed by those containing fluoride. Herbal products showed comparatively lesser activity in inhibiting the growth of S. mutans. Even though there are so many products available to curb the progression of dental caries in the population targeted, there is still an undoubted prevalence and incidence of caries among the general public. The need for a better alternative to help control dental caries is on the rise even today.