Identification and purification of a local isolate of infectious laryngotracheitis virus

Local infectious laryngotracheitis [ILT] virus strain was isolated on ECE for ten passages. The isolated virus produced characteristics pock lesions on chorio-allantoic membranes of the embryonated eggs, which increased in size and number when the number of passage increases. Moreover, the isolated virus was further propagated on CER and Vero cells where the virus gave high titers only when propagated on Vero cells. The virus was purified by sucrose gradient ultra centrifugation where a white band was collected at the end of the centrifugation cycle. The isolated ILT virus was identified by means of Electron Microscopy, Dot-ELISA and serum neutralization

Preparation of cell-culture inactivated infectious laryngotracheitis virus vaccine adjuvanted with nigella sativa oil

Isolated and identified infectious laryngotracheitis virus was inactivated by [BEI] and tested for its sterility, safety and potency then the inactivated ILT virus was mixed by addition of oil adjuvant, which consists of Nigella sativa and paraffin oil [PANISA oil] in the ratio of 1: 1. Two vaccines were made, the first where the aqueous phase to the oil phase was 1: 4 while the second vaccine where the ratio was 1: 2. Quality control tests were made such as drop test, safety and sterility test. The emulsion was oil in water, safe and free from bacterial, fungal and Mycoplasma contamination. Three experiments were made; the first where the vaccines were giving to chicken and the antibody pattern during 16 weeks were demonstrated using ELISA. The second experiment where comparison between the inactivated vaccines and the live attenuated vaccine was done. The third experiment included three successive challenges with four weeks intervals. Vaccines prepared adjuvanted with Nigella sativa oil produced higher humeral response than live attenuated vaccine