Studies on quality standard of PsL 5F injections / 中国中药杂志

<p><b>OBJECTIVE</b>To establish the quality standard of PsL injections containing mainly 5F (ent-11alpha-hydroxy-15-oxo-kaur-16-en-19-oic-acid).</p><p><b>METHOD</b>The identification of PsL was performed by thin-layer chromatography, and the content was determined by HPLC. The column was Hypersil C18 (4.6 mm x 250 mm, 5 microm), the mobile phase was the mixture of methane-water-acitic acid (55:45: 0.045) with a flow rate of 1.0 mL x min(-1), the detective wavelength was 254 nm, and the column temperature was maintained at 35 degrees C. The pH value and K+ content of the three batchs injection were determined with pH meter and flame photometric meter, and the contents of tannin, protein, oxalic acid salt and heavy metals were detected by deferent methods.</p><p><b>RESULT</b>The TLC method was suitable for the identification of PsL5F. The linearity for 5F was obtained over the range of 30-240 microg x mL(-1) (r = 0.999 8), the average recovery of 5F was 99.8%. The injections were of pH value range from 7.80 to 8.20, K+ contents less than 10 mmol x L(-1), and the contents of tannin, protein, oxalic acid salt and heavy metals were qualified with the Chinese pharmacopoeia, respectively.</p><p><b>CONCLUSION</b>It's sensitive and reliable that can be used as quality control methods of PsL5F injections.</p>

Effect of nobiletin on tubulin polymerization in vitro / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the possible mechanisms of nobiletin for anticancer by studying the inhibition effects of nobiletin on tubulin polymerization.</p><p><b>METHOD</b>In vitro nobiletin was added into the tubulin polymerization-depolymerization system and the absorption values were recorded at 350 nm under 37 degrees C.</p><p><b>RESULT</b>As compared with controls, the absorption values in reaction system decreased significantly in nobiletin treatment groups. When nobiletin final concentrations in reaction system were 5.0, 7.5, 10.0 and 12.5 micromol x L(-1), the maximum absorption values were 0 130, 0.109, 0.086 and 0.071 with 16.7%, 30.1%, 44.9% and 54.5% of inhibition rate, respectively. The results suggested that nobiletin could inhibit tubulin polymerization.</p><p><b>CONCLUSION</b>The inhibition effect of nobiletin on tubulin polymerization is the possible mechanism for anticancer.</p>

Protective effect of Armillaria mellea polysaccharide on mice bone marrow cell damage caused by cyclophosphamide / 中国中药杂志

<p><b>OBJECTIVE</b>To observe the effect of Armillaria mellea polysaccharide on mice bone marrow cells damage caused by Cyclophosphamide.</p><p><b>METHOD</b>Kunming purebred mice were used and stochastic divided into 5 groups: normal control group, positive control group (rhG-CSF 20 microg x kg(-1) x d(-1)), damage group of Cyclophosphamide (150 mg x kg(-1) x d(-1)), the protective group with A. mellea polysaccharide, low dose (25.0 mg x kg(-1) x d(-1)) and high dose (50.0 mg x kg(-1) x d(-1)). Positive control was s.c. rhGCSF 6 d and i.p. Cyclophosphamide 3 d. A. mellea polysaccharide was i.p. 8 d. and Cyclophosphamide i.p. 3 d. WBC, RBC, PLT, BMNC were counted in peripheral blood and bone marrow cells. The myelogram were analyzed in bone marrow.</p><p><b>RESULT</b>The WBC, RBC, PLT, BMNC of protective group and positive control group were higher than damage group (P < 0.01) significantly high dose group increaser than low dose group in protective group the numbed of Promyelocytic and lobulation nuclear of marrow.</p><p><b>CONCLUSION</b>A. mellea polysaccharide has preferably protective effect on damaging mice bone marrow cell caused by Cyclophosphamide.</p>