RESUMO
Objective To develop a rapid method for the identification of inactivated C.albicans by surface-enhanced Raman spectroscopy (SERS).Methods Live C.albicans cultures were exposed to heating, formaldehyde and fungicidal drug (amphotericin B).The corresponding SERS spectra were acquired for the investigation and comparison.Results The spectra acquired with three different inactivation methods exhibited similar features of dead C.albicans, which showed significant difference from the spectra of the live culture.Conclusion This SERS method can identify the inactivated C.albicans rapidly.Hopefully it will provide a convenient tool for quick identification of other inactivated pathogenic microorganisms.
RESUMO
Objective To establish a paper-based surface-enhanced Raman scattering (SERS) method for the detection of dyed K adsura longipedunculata Finet et Gagnep .Methods Supported silver nanoparticles on filter paper were synthesized simply by soaking method .In addition ,factors including enrichment ratio of silver nanoparticles ,the enhancement effect and sta-bility of the SERS device were investigated .Results The K adsura longipedunculata Finet et Gagnep ,dyed by Erythrosine or Acid Red at low concentration had been detected successfully .Conclusion Combined with the paper device and SERS ,the ap-proach was rapid and non-destructive which could be used to identification of dyed K adsura longipedunculata Finet et Gagnep .