RESUMO
To investigate the effect of three kinds of polymeric scaffolds on attachment, proliferation and differentiation of bone marrow mesenchymal stem cells, the cells were different polymeric scaffolds of PLA-PEG, PLA, PLGA, respectively. The proliferation of cell was evaluated by cell count; the attachment and morphology of BMSCs were observed by SEM; and differentiation was detected by alkaline phosphatase activity, fluorescence, and RT-PCR methods. Results showed that the cells in PLGA group spread better among BMSCs adhered to the three polymeric scaffolds. The activity of ALP was detected after 3 days culture in these three groups. There were no significant differences between PLA-PEG and PLGA groups, but the activity of ALP was higher than PLA group. The gene expressions of osteocalicin and collagen I were also observed in the early culture time. Calcium nodes formation in these polymeric scaffolds were detected. BMSC spreading first, then overlapping growth and secretion of matrix around the bottom and surface of scaffolds were observed through SEM. In summary, PLA-PEG and PLGA are better polymeric scaffolds for the bone tissue engineering, compared with PLA.
Assuntos
Células da Medula Óssea , Biologia Celular , Adesão Celular , Proliferação de Células , Ácido Láctico , Química , Células-Tronco Mesenquimais , Biologia Celular , Poliésteres , Química , Polietilenoglicóis , Química , Ácido Poliglicólico , Química , Polímeros , Química , Engenharia Tecidual , Alicerces TeciduaisRESUMO
0.05). ALP activity of the cells on deproteinized bone was higher than that on decalcified bone(P