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Objective] To investigate the relationship between the AGE-RAGE and Qi and Yin deficiency and blood stasis of pathogenesis in diabetic nephropathy(DN).[Methods] The model was established by intravenous injection low-dose streptozotocin(STZ, 30 mg·kg-1) after having the high fat/high glucose diets for one month. And then that were divided into DN group, Tangshenfang low, medium, high groups, Valsartan group. Fasting blood-glucose (FBG) and Creatinine clearance(Ccr) were monitored. The morphological changes of renal tissue were examined under microscopy on sections stained with PAS. The RAGE mRNA and protein expression were determined by Real-time PCR and Western blot respectively;the urine album(U-alb) and content of AGE in serum were determined by ELISA. [Results] The model group rats acted obviously as deficiency of both Qi and Yin and obstruction of collaterals. compared with normal group, FBG, urinary albumin excretion rate and the content of AGE, RAGE expression were markedly increased; Ccr was markedly decreased in DN model group;blood viscosity and whole blood reduction viscosity were increased. FBG, Urinary albumin excretion rate and the content of AGE, RAGE expression were markedly decreased, Ccr was markedly increased by the intervention of Tangshenfang and valsartan. Blood viscosity and whole blood reduction viscosity were decreased; it was obviously and positively correlated between AGE with RAGE expression and U-alb, excretion rate and negatively correlated with the Ccr, r value was-0.456. [Conclusion] The early DN model rats were in the pathogenesis of Qi and Yin deficiency and blood stasis. And the AGE-RAGE may have close relationship with the pathogenesis of Qi and Yin deficiency and blood stasis.
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AIM:To investigate the effects of silent information regulator 1 (SIRT1) on high glucose-induced acetylation of NF-κB p65 subunit and its protective role in rat mesangial cells .METHODS:Rat mesangial cells were cul-tured in DMEM supplemented with 10% FBS and were divided into control group , mannitol group , high glucose group , resveratrol group and SIRT1 RNAi group.The cell viability was determined by MTT assay .The mRNA expression of SIRT1, monocyte chemoattratant protein 1 (MCP-1), vascular cell adhesion molecule 1 (VCAM-1-1), tumor necrosis fac-torα( TNF-α) , transforming growth factor β1 ( TGF-β1 ) was analyzed by real-time quantitative PCR .The protein expres-sion of SIRT1 and the acetylation of NF-κB p65 subunit were determined by Western blotting .The protein concentrations of MCP-1, VCAM-1, TNF-α, TGF-β1 and malondialdehyde (MDA) were detected by ELISA.RESULTS:The cell viabili-ty, superoxide dismutase (SOD) activity, and the expression of SIRT1 at mRNA and protein levels were decreased by high glucose treatment as compared with control group .The acetylation of NF-κB p65 subunit was significantly increased after interfered with high glucose , resulting in the increase in the secretion of MCP-1, VCAM-1, TNF-αand TGF-β1 .Resvera-trol decreased high glucose-induced acetylation of NF-κB p65 subunit.However, silencing SIRT1 significantly enhanced the acetylation of NF-κB p65 subunit and the expression of MCP-1, VCAM-1, TNF-αand TGF-β1 .CONCLUSION:SIRT1 remarkably inhibits the inflammatory reactions by deacetylating NF-κB p65, suggesting that SIRT1 is a possible tar-get for preventing diabetic nephropathy .
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[Objective]To observe the curative effect of the Tangshen Particle on diabetes.[Methods] ICR mice were induced for diabetes by Alloxan intravenous injection,take Tangshen Particle and Xiaoke pill as the comparison group,observe each group’s glucose,lipid,renal index,urea,creatinine.[Results] The Tangshen Particle was able to decline glucose,lipid,renal index,urea,creatinine on diabetic mice,but the effect on high dose group was most obvious.[Conclusion] Tangshen Particle has good glucose-lowering and lipid-lowering effect on diabetic mice,and it has preventive and therapeutical effects on diabetic nephropathy .
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BACKGROUND: Glomerular mesangial cell (GMC) is the chief target cell of diabetic nephrosis. A series of functional alternations on mesangial cell (MC) in high glucose condition results in constant development of the disease. Intervention of Chinese herb is very significant in retarding the progression of diabetic nephrosis.OBJECTIVE: To probe into GMC proliferation in high glucose condition and the effects of serum with tangshen tang contained on it.DESIGN: Controlled experiment was designed.SETTING: Laboratory Room of Second Affiliated Hospital to Zhejiang College of Traditional Chinese Medicine.MATERIALS: MCs of SD rats were taken as experimental objects collected from Internal Department of Kidney of Shanghai First People's Hospital in August 2003.Totally 45 SD rats of clean grade were employed from Experimental Animal Center of Zhejiang College of Chinese Medicine, of either sex,weighted with (180±20)g in October 2003 and they were prepared as the animals with herb-contained serum. Tangshen tang [composed of dahuang (Rhubarb), taoren (Semen Persicae), chong (insects), huangqi (Radix Astragali seu Hedysari), digupi (Cortex Lycii Radicis), huanglian (Rhizoma Coptidis),etc.] was concentrated as 3 g/mL after decoction and infiltration at ratio.METHODS: SD rats were randomized as normal group, tangshen tang group and captopril group, 15 rats in each. The serums with tangshen tang and captopril of different doses were applied in MCs. In normal group,gastric perfusion of equal volume of distilled water was applied everyday.Trypan blue staining and MTT method were used respectively to assay the effects on MC toxicity and proliferation after medication.MAIN OUTCOME MEASURES: Activity of GMC in rats after stimulation of medical-contained serums and cellproliferation.liferation was inhibited (P ＜ 0.05 , vs blank group) in constant high-glucose condition and the inhibited proliferation due to high glucose was reversed to various extents after the intervention of tangshentang and captoter the intervention of medical-contained serums on GMCs in rats in every group (cell activity was up to over 94% and P ＞ 0.05, vs blank group).CONCLUSION: High glucose inhibits MC proliferation and tangshentang stimulates MC proliferation.
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Drug pairs,an important ingredient in prescription compatibility,is the bridge between Chinese materia medica and prescriptions.This paper discusses the effect of drug pairs in treating diabetes and its complications,thus,studys the effective prescriptions and methods of treating diabetes and its complications.
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The main pathogenesis of diabetes is that spleen weakness losing transports induces nutrient substance metabolic disturbance in the body,so as results the patient's GLU elevated.Spleen weakness is the foundation of diabetes and its complications.In the therapy it should be treated from the spleen using activating spleen-energy method.In this article it proposed that in clinic we could apply the five methods in terms of dissipating dampness,dispersing the depressed liver-energy,promoting blood flow,eliminating phlegm and warm the kidney to activate spleen-energy in treating diabetes and its complications.
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Objective:To explore the effect of modifi ed Didang Decoction on AngII in insulin resistance rats.Methods:The insulin resistance rats were randomly divided into the control group,the model control group,the high,medium and low dosage of modif ied Didang decoction groups and Wendiya group.Radioimmunity assay was used to observe the effect of modif ied Didang Decoction on the AngII in rats.Results:Compared with the model control group,the AngII signifi cantly decreased in the medium and high dose of modifi ed Didang Decoction groups(P
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Intervention and prevention of IGT population is crucial for reducing high incidence of diabetic.This paper analyzed the insufficiency of the existing IGT intervention and prevention models by introduing the concept of human health view of ecological theory,and emphasized the close relationship between IGT population and environment.The ecological explanation of TCM etiology of IGT and the ecological evaluation standard of IGT intervention and prevention model were also discussed.This paper further indicated that only by focusing on adapting to the living environment,applying the concepts of "integrated ecosystem management" can we successfully construct a sustainable IGT prevention and intervention model and effectively promote the model into a large-scale community.
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Objective: To investigate the effect of Tangshentang on the expression of LOX-1 and secretion of TGF-?1,FN,ColⅣ in cultured rat masengial cells(RMCs) induced by ox-LDL and to explore its molecular mechanism of preventing and treating diabetic nephropathy.Methods: The cultured RMCs were divided into control group,ox-LDL group,Tangshentang group(respectively high,medium and low group)and rosiglitazone group.The mRNA expression was tested by RT-PCR.Synthesis of TGF-?1,FN,ColⅣ in cultured RMCs were determined by ELISA methods.Results: Tangshentang drug serum significantly attenuated up-regulation of TGF-?1,LOX-1 mRNA expression and synthesis of TGF-?1,FN,ColⅣ in a dose-dependent manner,with the peak at middle concentration,in RMCs stimulated by ox-LDL(50?g/ml).Conclusion: Tangshentang drug serum may protect kidney from injury by ox-LDL via the decreased expression of LOX-1 to reduce the uptake of ox-LDL and subsequently inhibiting TGF-?1 secretion,as well as the deposition of FN,ColⅣ.