RESUMO
Objecave To explore the protective effect of active component of Eclipta on hepatic injury induced by Concanavalin A(ConA)in mice. Methods Mouse hepatic injury models were established by injection of ConA(15 mg/kg)via tail vein.The effect of Eclipta extracts on serum alanine aminotransferase (ALT) and liver histology in both normal mice and hepatic injury mice was examined.A pure compound was obtained by means of bioactivity-guided isolation,and IR,~(13)C-NMR, ~1H-NMR,~1H-~(13)C HMBC and ~1H-~(13)C HMQC were used for structural identiffcation. Results Eclipta extracts decreased serum ALT in hepatic injury mice induced by ConA.and demonstrated anti-apoptosis effect induced by ConA in hepatoeytes.Wedelolaetone,a eoumarin,was isolated from Eclipta,and its structure was identified on the basis of spectroscopic analysis.Wedelolactone inhibited ConA-induced T cell proliferation selectively,and completely antagonized the effect of ConA at the concentration of 10μg/mL. Conclusion Eclipta herb has hepatoprotective effect.Wedelolactone,the anti-inflammatory and anti-apoptosis active component of Eclipta,might serve as a lead compound for developing new antiinflammatory drugs.
RESUMO
Objective To evaluate the strengthening and restoring effects and hepatoprotective effect of Eclipta extracts. Methods Sixty Kunming mice were randomly divided into control group, low-dose Eclipta group, high-dose Eclipta group, cyclopho9phamide(CY) group, low-dose Eclipta CY group and high-dose Eclipta CY group(n = 10). Eclipta 8 g·kg~(-1)d~(-1) or 4 g·kg~(-1) · d~(-1) were administered by oral gavage for 7 d. Immunosuppression mouse models were established by intraperitoneal injection of 0. 05 g/kg CY on the fourth day of administration. The effects of Eclipta extracts on thymus index, spleen index and carbon clearance index in both normal mice and immunosuppression mice induced by CY were tested, and those on proliferation of lymphocyte and T cells induced by ConA were examined. Based on these data, strengthening and restoring effects of Eclipta were observed. Besides, 70 Kunming mice were randomly divided into control group, low-dose Eclipta group, high-dose Eclipta group, CCl_4 group, DDB group, low-dose Eclipta CCl_4 group and high-dose Eclipta CCl_4 group. 20 g·kg~(-1)·d~(-1) and 10 g·kg~(-1) ·d~(-1) Eclipta were administered by oral gavage for 7 d. Hepatic injury mouse models were established by intraperitoneal injection of 10 mL/kg CCl_4 2 h after the last administration. The effects of Eclipta extracts on serum ALT, MDA, TP and T-Bil levels in both normal mice and hepatic injury mice induced by CCl_4 was tested, and those on the viability of the primary culture hepatocytes were determined. Based on these data, hepatoprotective effect of Eclipta was observed. Results Eclipta extracts increased thymus index and carbon clearance index in both normal mice and immunosuppression mice induced by CY, promoted proliferation of lymphocyte and proliferation of T cells induced by ConA, reduced serum ALT and MDA levels in hepatic injury mice, and improved the viability of the primary culture hepatocytes. Conclusion Eclipta herb has strengthening and restoring effects and hepatoprotective effect.