E3 ubiquitin ligase alleviates DSS-induced colitis via regulating NLRP3 inflammasome activation / 公共卫生与预防医学

Objective To investigate the role of TRIM65 on DSS induced colitis and the underlying molecular mechanisms. Methods Trim65+/+ and Trim65-/- mice were administered with 3% (w/v) DSS in their drinking water for 5 consecutive days and then were switched to sterile water for 2 days. DSS treated mice were monitored daily for the clinical symptoms (bodyweight, stool consistency and rectal bleeding score). Mice were sacrificed on day 7 to measure colon length. Colon homogenates were collected to measure MPO activity and detect cleaved caspase-1 and mature IL-1β by Enzyme linked immunosorbent assay (ELISA) and Western blot. Trim65-/- mice were intraperitoneally injected with NLRP3 inflammasome inhibitor MCC950, and were given the above treatment to determine the effect of MCC950 on colitis in Trim65-/- mice. Results The results showed that deletion of Trim65 significantly enhanced weight loss and colon shortening in DSS mice, increased disease activity index and histopathological score, induced the activity of MPO, and promoted the F4/80+ immune cell infiltration, the activation of caspase-1 and the secretion of mature IL-1 in the colon of DSS mice. The NLRP3 inflammasome inhibitor MCC950 alleviated DSS induced colitis symptoms and inflammation levels in trim65 deficient mice. Conclusion TRIM65 plays an anti-inflammatory role in DSS induced colitis mice by inhibiting the activation of NLRP3 inflammasome.

Analysis of mineral contents in the colostrum and peripheral blood and their correlation in pregnant women in Beijing / 公共卫生与预防医学

Objective To investigate the mineral contents of colostrum and peripheral blood and their correlation in pregnant women in Beijing. Methods A total of 80 pregnant women who gave birth in the Affiliated Fuxing Hospital of Beijing Capital Medical University in May 2019 were selected as research subjects, and their colostrum and peripheral blood samples were collected. The contents of 46 minerals in the colostrum and peripheral blood samples were determined by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) and Atomic fluorescence spectrum (AFS). The correlation between the contents of minerals in the colostrum and peripheral blood was analyzed by SPSS 21.0. Results Among the 46 minerals detected, the minerals with higher contents in the peripheral blood were sodium, potassium, iron, magnesium, calcium, zinc, rubidium, copper, aluminum and selenium. The minerals with higher colostrum contents included potassium, sodium, calcium, magnesium, zinc, iron, rubidium, copper, strontium and aluminum. The contents of calcium, strontium, cesium, molybdenum, cobalt, uranium and thorium in the colostrum were significantly higher than those in the peripheral blood (P<0.05). There were significant correlations in the contents of sodium, rubidium, selenium, strontium, cesium, arsenic, mercury and cadmium between the colostrum and peripheral blood (P<0.05). In addition, there were significant correlations between some minerals in the colostrum, for example potassium vs manganese (r=-0.236, P=0.043), rubidium (r=0.816, P<0.001) and magnesium (r=0.400, P<0.001); sodium vs selenium (r =0.509, P<0.001), cerium (r=0.353, P=0.002), praseodymium (r =0.337, P=0.003) and thulium (r=0.331, P=0.004); calcium vs iron (r=0.251, P=0.031) and strontium (r =0.365, P=0.001); magnesium vs cesium (r=0.314, P=0.006) and copper (r =0.395, P=0.001); zinc vs selenium (r=0.310, P=0.007), cerium (r=-0.229, P=0.050), gadolinium (r=-0.372, P=0.001) and yttrium (r=-0.380, P=0.001); rubidium vs titanium (r=-0.413, P<0.001); copper vs cesium (r=0.275, P=0.018); strontium vs uranium (r=0.439, P<0.001); cadmium vs molybdenum (r=0.379, P =0.001). Conclusion The correlations of the mineral contents between the colostrum and peripheral blood suggested that some mineral elements could be accumulated from blood to breast milk through various transport mechanisms. The correlations between the mineral elements in the colostrum showed that there were some interactions between these elements, indicating that maintaining the dynamic balance of mineral elements in human milk is very important for the health of their offspring.

Effects of calcium supplementation during the pregnancy and early infancy stage on the body mass index and gut microbiota in the infants / 中华预防医学杂志

Objective@#To investigate the effects of calcium supplementation during the pregnancy and early infancy stage on body mass index (BMI) and gut microbiota in the infants.@*Methods@#A total of 1 752 healthy pregnant women and their infants (breast feeding) in two maternal and child health care hospitals of Beijing were chosen as the subjects in this study from May to October 2016. Questionnaires were used to obtain the general information and supplementation of calcium and vitamin D in mothers and their infants. The body length and weight of infants at birth and 6 months were recorded to calculate the BMI. The random number table method was used to randomly select 40 infants from each group for gut microbiota analysis (If less than 40 infants were all included in this study, 23 infants in the pregnancy and early infancy would be all treated with calcium supplements. There were 6 infants who was not added calcium during the pregnancy but added in the early infancy). Then it was compared that the effects of calcium supplementation during the pregnancy and early infancy on the BMI and gut microbiota composition of infants were determined at birth and 6 months.@*Results@#Compared to the group with no calcium supplementation during the pregnancy ((12.76±1.23), (17.68±0.76)kg/m2), the BMI of infants at birth and 6 months in the group with calcium supplementation during the pregnancy ((13.51±0.47), (17.91±0.23)kg/m2) were significantly higher(P<0.05). In the group with maternal calcium supplementation, the BMI at 6 months ((18.63±0.52)kg/m2), BMI increment ((5.71±0.54)kg/m2) and the content of lactobacillus (21.04%±3.68%) in the only calcium supplementation subgroup in the early infancy were higher than those in only vitamin D supplementation subgroup ((17.69±0.89) kg/m2, (4.17±1.01) kg/m2 and 12.28%±3.86%) (P<0.05). In the group without maternal calcium supplementation, the content of lactobacillus (20.15%±4.87%) in the only calcium supplementation subgroup were also higher than those in only vitamin D supplementation subgroup (14.64%±3.71%) (P<0.05).@*Conclusion@#Appropriate calcium supplementation during the pregnancy is good for the growth and development of the fetus. Calcium supplementation in the early infancy could increase the BMI of infants, and promote the growth of intestinal lactobacillus.

Effect of docosahexenoic acid supplementation on infant's growth and body mass index during maternal pregnancy / 中华流行病学杂志

Objective To investigate the effects of docosahexenoic acid (DHA) supplementation on infant's growth and BMI during pregnancy.Methods A total of 1 516 healthy pregnant women delivered their babies in two maternal and child health care hospitals in Beijing and were chosen as the subjects in this cohort study from May to October 2015.Self-developed questionnaires were used to gather general information of the subjects,including age,height,weight,weight gain during pregnancy,delivery mode,DHA supplementation etc.,before giving birth.Information on body length,weight,head circumference and BMI at birth and 6 months postnatal,of the infants were recorded.Breast milk was collected to test the fatty acid profiles by using the gas chromatography (GC) method at one to three months postnatally.Results The overall rate of DHA supplementation was 47.76％ among the pregnant women,in which introduction of DHA from the early and second stage of the pregnancy accounted for 49.31％ and 39.64％ respectively.When DHA supplementation began from the early pregnant stage,the DHA concentration showed an increase in the milk (P＜0.05),whereas the supplementation began from the second and third stages did not affect the milk DHA concentration (P＞0.05).Higher height and lower BMI were seen in the infants at birth and 6 months in the supplementation group when comparing to the non-supplementary group (P＜0.05),with the greatest effects noticed in the earliest supplementation group.Specifically,the head circumference appeared larger from the early pregnant stage in the DHA supplementary group,than that in the non-supplement group (P=0.001).The increment of head circumference was larger than that in the other groups when the infants were 6-month old (P＜0.01).Results from the partial regression analysis showed that during pregnancy,there were positive correlations between DHA supplementation and height (r=0.324,r=0.216),head circumference (r=0.221,r=0.302) as well as the increment of head circumference (r=0.276) at birth and 6 months (P＜0.05).Whereas,a negative correlation was shown between DHA and the infants' BMI (r=-0.310,r=-0.371) (P＜0.05) when supplementation was given during maternal pregnancy.Conclusions When DHA supplementation program was carried out during maternal pregnancy,it could increase the height and head circumference and inhibit the rapid increase of BMI in the infants BMI.Our findings seemed helpful in promoting brain development and preventing the childhood obesity.

Effect of docosahexenoic acid supplementation on infant's growth and body mass index during maternal pregnancy / 中华流行病学杂志

Objective To investigate the effects of docosahexenoic acid (DHA) supplementation on infant's growth and BMI during pregnancy.Methods A total of 1 516 healthy pregnant women delivered their babies in two maternal and child health care hospitals in Beijing and were chosen as the subjects in this cohort study from May to October 2015.Self-developed questionnaires were used to gather general information of the subjects,including age,height,weight,weight gain during pregnancy,delivery mode,DHA supplementation etc.,before giving birth.Information on body length,weight,head circumference and BMI at birth and 6 months postnatal,of the infants were recorded.Breast milk was collected to test the fatty acid profiles by using the gas chromatography (GC) method at one to three months postnatally.Results The overall rate of DHA supplementation was 47.76％ among the pregnant women,in which introduction of DHA from the early and second stage of the pregnancy accounted for 49.31％ and 39.64％ respectively.When DHA supplementation began from the early pregnant stage,the DHA concentration showed an increase in the milk (P＜0.05),whereas the supplementation began from the second and third stages did not affect the milk DHA concentration (P＞0.05).Higher height and lower BMI were seen in the infants at birth and 6 months in the supplementation group when comparing to the non-supplementary group (P＜0.05),with the greatest effects noticed in the earliest supplementation group.Specifically,the head circumference appeared larger from the early pregnant stage in the DHA supplementary group,than that in the non-supplement group (P=0.001).The increment of head circumference was larger than that in the other groups when the infants were 6-month old (P＜0.01).Results from the partial regression analysis showed that during pregnancy,there were positive correlations between DHA supplementation and height (r=0.324,r=0.216),head circumference (r=0.221,r=0.302) as well as the increment of head circumference (r=0.276) at birth and 6 months (P＜0.05).Whereas,a negative correlation was shown between DHA and the infants' BMI (r=-0.310,r=-0.371) (P＜0.05) when supplementation was given during maternal pregnancy.Conclusions When DHA supplementation program was carried out during maternal pregnancy,it could increase the height and head circumference and inhibit the rapid increase of BMI in the infants BMI.Our findings seemed helpful in promoting brain development and preventing the childhood obesity.

Beneficial effects of n-3 polyunsaturated fatty acids on child development and health / 中华实用儿科临床杂志

n -3 polyunsaturated fatty acids (n -3 PUFAs),essential fatty acids for human body,have varied biological activities.They are absolutely or relatively deficient in modern human diets.Supplementation of n -3 PUFAs in early life is important for the optimal growth and development in fetus,infants and children,and well as for prevention of chronic non -communicable diseases in later life,such as attention deficient hyperactivity disorder,allergic diseases, asthma,hyperlipidemia and non -alcoholic fatty liver disease.The human body can synthesize eicosapentaenoic acid (EPA)and docosahexaenoic acid (DHA)with linolenic acid,but the ability is limited.Therefore,intake of pre -formed EPA and DHA is needed for body requirement.For pregnant women and infants,supplementation of DHA and EPA with a ratio above 51 is suggested.In clinic,n -3 PUFAs have been applied for treatment of chronic non -com-municable diseases and proved to be beneficial in relief of symptoms.

Role of dextran sulfate in the catabolism of lipid emulsions / 中华临床营养杂志

ObjectiveTo investigate the effects of scavenger receptor A on the catabolism of lipid emulsions and further to see if it differently affects long-chain triglyceride (LCT) and fish oil (FO) emulsions. MethodsA total of 24 C57BL/6J female mice, 10 to 12 weeks old, were randomly divided into 4 groups with 6 mice in each group. Two groups of mice were intravenously injected with dextran sulfate ( DexSO4 ) ( 1 mg/mouse) followed by intravenous injection of [1α, 2α(n)-3H] cholesteryl oleoyl ether [(3H)CEt] labelled LCT or FO emulsions (0.4mg triglycerde/mouse) at 2 minutes respectively, and other two groups were injected by saline as controls before injection of (3H)CEt labelled LCT and FO emulsions. Then, blood was drawn at fixed intervals to measure the radioactivities and the emulsion's fractional catabolic rates (FCR) were calculated. With the same procedures above mentioned, non-radiolabelled LCT and FO emulsions were intravenously injected to mice to determine liver uptake of lipid emulsions under electromicroscopy. Finally, THP1 cell line was used to examine the effects of DexSO4 on cell uptake of LCT and FO emulsions in vitro. ResultsPre-injection of DexSO4 to mice decreased the FCR of both LCT and FO emulsions at 72.38％ and 47.38％ respectively, as compared to controls ( P =0.020 ). Electromicroscopy showed that pre-injection of DexS04 decreased the uptakes of LCT and FO emulsions by Kupffer cells and sinusoidal endothelial cells similarly. In hepatocytes, no lipid droplets existed in mice with LCT emulsion injection, whereas some lipid droplets were still shown in mice with FO emulsions but with less quantities compared to control mice.In vitro, addition of DexSO4 to medium decreased THP1 cell uptakes of LCT and FO emulsions ( P =0.003 and 0.008) by 30.74％ and 41.60％ respectively. However, no differences were found in the effects of DexS04 on cell uptakes between LCT and FO emulsions ( P =0.080). ConclusionScavenger receptor A plays important roles in catabolism of lipid emulsions to some extent, and it's effects on FO emulsions may be less than LCT emulsions.