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Objectives To explore the route of ESBL producing bacteria in neonatal faeces, and to investigate the gene and drug resistance of ESBL producing bacteria in intestinal tract of neonates. Methods Fecal samples of healthy newborns and their mothers were collected, and bacterial cultures were carried out using selective ESBL medium. The positive strains were identified by Time-of-flight mass spectrometry. ESBL genotyping and resistance gene detection were performed by whole genome sequencing technique. Results In 146 neonatal fecal specimens, the positive rate of ESBL producing bacteria was 8.90％,and the positive rate in the first time stool was 3.23％. Seventy-two hours after birth, the positive rate of fecal ESBL producing bacteria was 13.10％. Among the 13 ESBL producing strains, there were 9 strains of CTX type, 3 strains of TEM type and 1 strain of SHV type. Nine strains of CTX include five types such as CTX-M-24, CTX-M-18, CTX-M-27, CTX-M-42 and CTX-M-15. The positive rate of ESBL producing bacteria was 21.6％ in 167 mothers' fecal specimens. The ESBL genotype included 24 strains of CTX type, 6 strains of TEM type, 4 strains of SHV type and 2 strains of QnrS type. Twenty-four strains of CTX include CTX-M-24, CTX-M-14, CTX-M-18, CTX-M-27, CTX-M-42 and CTX-M-15. There were 2 or 3 ESBL genotypes in 12 maternal and neonatal specimens. It was detected to have 6 types of resistance gene such as aadA5, strA, strB, sul1, sul2 and dfrA17 in 49 strains of ESBL producing bacteria in maternal and neonatal strains. Resistance genes were exactly the same in the neonates as in mothers who were detected to have ESBL producing bacteria. A variety of resistance genes were detected in feces in 7 neonates and 23 mothers. Conclusions The neonates in hospital may be detected to have ESBL produing bacteria in the intestinal tract at the same time as their mothers or separately. However, there are many ways for neonates to have ESBL producing bacteria in intestinal tract. There are many genotypes and resistance genes of ESBL producing bacteria.
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Parenting stress is regarded as a criterion to evaluate the parent's mentation of children with chronic illness,it not only influence parent's care of children,but also decrease the quality of children's life.Si multaneously,it is a main reason of family imbalance.This paper introduces the definition of parenting stress and evaluation tool summarizes the cunent situation of research in abwad,provides a foundation in order to promote the research of parenting stress among caregivers of children with chronic illness.
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Objective To explore the relationship between emotional intelligence and job performance by investigating the level of them in pediatric nurses,and to provide a basis for improving job performance and the implementation of“quality care.Methods 335 pediatric nurses were investigated by using emotional intelligence scale and job performance scale.And the result of the investigation was analyzed statistically.Results The average score of the emotional intelligence of the pediatric nurses were (3.63±0.52),the highest score was interpersonal latitude (3.87± 0.69),the lowest score was management of emotions latitude (3.25±0.90); titles,the form of post and degree of nurses had a certain influence on the emotional intelligence.The score of job performance was(3.65±0.75).By Pearson correlation analysis,the emotional intelligence score and job performance score were obtained a significant positive correlation,self-motivation latitude and job performance were the most related,the management of emotional latitude job performance was the lowest.Conclusions Nursing managers should take corresponding measures to improve nurses' emotional intelligence and job performance levels,and promote the implementation of quality care effectively.