[Compraision of urease test with Giemsa staining for detection of Helicobacter pylori infection in patients with gastric ulcer and gastritis]

Background and Objective: Helicobacter pylori are one of the most common gastrointestinal bacterium infections. This study was done to compare the urease test with Giemsa staining to detection of Helicobacter pylori infection in patients with gastric ulcer and gastritis

Methods: This descriptive- analytic study was conducted on 601 patients' diagnosed with gastric ulcer and gastritis, whom under went endoscopy in 5th Azar hospital, Gorgan, Iran during 2008-12. Rapid urease test was immediately taken during endoscopy. Pathological examination, Giemsa staining on paraffin blocks and haematoxylin and eosin stain were done to assess either presence or absence of Helicobacter pylori

Results: Based on biopsy results, Gastritis and gastric ulcer were diagnosed in 80.69% and 19.3%, respectively. In gastritis and gastric ulcer patients, there was a significant differences between urease test [91.5% and 90%, respectively] in comparison with Giemsa staining [91.5% and 90%, respectively] [P<0.05]

Conclusion: The study revealed that in primary diagnosis and screening of role of Helicobacter pylori in patients with gastric ulcer and gastritis, urease test is suitable than giemsa staining

[Assessment of babA2 and hsp genotype frequency in helicobacter pylori specimens isolated from digestive disorders patients]

Helicobacter pylori is the main gastric pathogen in human. BabA2 and Hsp genotypes are essential for enormous clinical outcomes in gastreodeoneal and dyspepsia. This study was done to determine the assessment of babA2 and hsp genotype frequency in Helicobacter pylori specimens isolated from digestive disorders patients. This descriptive-analytic study was carried out on 80 digestive disorders patients in 5th hospital, Gorgan, northern Iran. Stomach specimen biopsy was taken by a gastroenterologist. Urease test, histopathologic assessment and DNA extraction were performed. The frequency of babA2 and hsp geno-types was determined using poly merase chain reaction. In 80 affected patients with H.pylori, 36, 18 and 26 patients were found to suffer from gastritis, stomach cancer and stomach ulcer, respectively. 51 specimens [63%] were positive babA2 genotype. 49 specimens [61%] were positive hsp genotype. No significant relationship was found between babA2 and hsp geno types with stomach diseases. In spite of positive babA2 and hsp genotype in isolated Helicobacter pylori speicments from digestive disorders patients, this finding was not correlated with type of digestive disorders