RESUMO
The antidiabetic effect of a dimethoxy derivative of perlargonidin 3-O-alpha-L rhamnoside (250 mg/kg, single dose study and 100 mg/kg/day long term study) isolated from the bark of Ficus bengalensis Linn. has been compared with that of glibenclamide (2 mg/kg and 0.5 mg/kg/day respectively) in moderately diabetic rats. The single dose glycoside treatment decreased fasting blood glucose by 19% and improved glucose tolerance by 29%. The corresponding effects of glibenclamide were 25% and 66% respectively over the control values. On one-month treatment the fasting blood glucose levels went down almost to half of the pretreatment levels in both the groups and their glucose tolerance improved by 41% in glibenclamide group and by 15% in glycoside treated group. Urine sugar decreased to traces in both the groups and they appeared healthy. In vitro studies showed that insulin secretion by beta-cells was more in presence of the pelargonidin derivative than in presence of a leucocyanidin derivative, reported to be a good anti-diabetic agent.
Assuntos
Animais , Antocianinas , Glicemia/análise , Diabetes Mellitus Experimental/tratamento farmacológico , Flavonoides/farmacologia , Glibureto/farmacologia , Glicosídeos/farmacologia , Hipoglicemiantes/farmacologia , Insulina/metabolismo , Extratos Vegetais/farmacologia , Ratos , Ratos Sprague-DawleyRESUMO
(-)Epicatechin, an active principle in the water extract of the bark of Pterocarpus marsupium increases the cAMP content of the islets which is associated with the increased insulin release, conversion of proinsulin to insulin and cathepsin B activity. The response of the islets to the (-)epicatechin stimulation is more pronounced in immature (one month old) than in mature (12 month old) rats.
Assuntos
Envelhecimento/metabolismo , Animais , Catequina/farmacologia , AMP Cíclico/metabolismo , Insulina/metabolismo , Ilhotas Pancreáticas/metabolismo , Masculino , Proinsulina/metabolismo , RatosAssuntos
Animais , Glicemia/metabolismo , Catepsina B , Catepsinas/metabolismo , Clorpropamida/farmacologia , Índia , Pâncreas/enzimologia , Plantas Medicinais , Ratos , SementesRESUMO
Α simple procedure for the isolation of cathepsin B from bovine pan creas employing ammonium sulphate fractionation, DEAE cellulose chromatography and Sephadex G 200 gel filtration is described. The purified enzyme gave a single band on polyacrylamide gel electrophoresis. The molecular weight as determined by gel filtration of the enzyme was 26,850. Its Km and Vmax values were 12·8 mM and 0·303 μmol/min/mg, respectively. The Ki for iodoacetamide was 0·16 mM.