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1.
Southeast Asian J Trop Med Public Health ; 1984 Sep; 15(3): 313-6
Artigo em Inglês | IMSEAR | ID: sea-32458

RESUMO

Four of the five veterinary E. coli strains, which were unable to transfer their antibiotic resistance by conjugation, were found to harbour plasmids. Evidence from transformation, agarose gel electrophoresis and curing experiments showed that in strains KE-3, KE-4 and KE-14 a nonconjugative R plasmid carried the gene for resistance to tetracycline. The plasmids in KE-9 were cryptic.


Assuntos
Animais , Antibacterianos/farmacologia , Conjugação Genética , Eletroforese em Gel de Ágar , Escherichia coli/efeitos dos fármacos , Malásia , Fatores R , Tetraciclina/farmacologia , Transformação Bacteriana
2.
Southeast Asian J Trop Med Public Health ; 1984 Mar; 15(1): 37-43
Artigo em Inglês | IMSEAR | ID: sea-33376

RESUMO

Fifteen independent E. coli strains of avian, bovine and porcine origin in Peninsular Malaysia were tested for antibiotic resistance and conjugative R plasmids. Eight (53%) isolates were found to be antibiotic resistant. Among them, 37.5% were mono-resistant and 62.5% were resistant to three or more antibiotics, i.e., multi-resistant. All of them were resistant to Tc and sensitive to Gm and Nx. Three of the eight antibiotic resistant strains were able to transfer all or part of their resistance to an E. coli K12 recipient by conjugation. The transfer frequencies of Km, Sm and Tc resistance of the three donors varied between 4.5 X 10(-8) to 6.8 X 10(-7). Analysis of the plasmid profiles of all the three donors and their respective transconjugants after agarose gel electrophoresis provided conclusive evidence that the transferable resistance traits were plasmid-mediated.


Assuntos
Animais , Antibacterianos/farmacologia , Bovinos , Conjugação Genética/efeitos dos fármacos , Resistência Microbiana a Medicamentos , Escherichia coli/efeitos dos fármacos , Infecções por Escherichia coli/tratamento farmacológico , Malásia , Plasmídeos/efeitos dos fármacos , Aves Domésticas , Suínos
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