RESUMO
A simple, specific, accurate and precise new high performance liquid chromatographic method was developed for the estimation of irbesartan in bulk and its developed dosage form. The mobile phase containing acetonitrile: Phosphate buffer pH 3.5 in proportion of 50:50 v/v was employed with flow rate of1.0 ml/min and eluting medium was monitored at 240 nm. The method was validated for linearity, accuracy, precision, limit of detection, limit of quantification and robustness for irbesartan. A linear response was observed in the range of 5- 40µg/ml. Linear regression of absorbance on concentration gave equation y = 101.9x + 195.3 with a regression co-efficient r2 =0.993. The method was validated for different parameters as per the ICH guidelines. The degradation studies were carried out by using the developed method. Thus the method was found to be useful for the determination of irbesartan in bulk as well as for dosage forms.
RESUMO
Introductions of high yielding inorganic fertilizer are not cheap and leave behind a large amount of dry matter for disposal. Thus there is a need to utilize these plant residues to save fertilizers and maintain a satisfactory level of soil fertility. Hence, with the view of in-situ manuring as well as to recycle the agricultural residue, pot experiments were conducted to study the effect of wheat husk amendments on soil microbial population and seedling vigour index (SVI) of wheat. Results of pot experiment showed the improvement in soil fungal population followed by bacterial and actinomycetal counts. Statistical significant increase in plant growth was also recorded as compared to unamended control, which indicates the practical possibility in utilizing wheat husk for improvement is soil fertility