Fixation of T allele in G>T Polymorphism in Exon 7 Region of Secreted Phosphoprotein 1 (SPP1) Gene in Indian Cattle Breeds

Secreted phosphoprotein 1 (SPP1) is popularly known as osteopontin (OPN), which plays an important role in initiation and maintenance of pregnancy, as well as in the development of the fetus and milk production. In the present study, investigation of G>T polymorphism in exon 7 region of SPP1 gene was undertaken in 147 Sahiwal and Hariana cattle maintained at Livestock Farm Complex (LFC), DUVASU, Mathura using HpyCH4IV/PCR-RFLP assay. Amplification of SPP1 exon 7 region revealed 204 bp product and HpyCH4IV restriction digestion screening showed monomorphic pattern. Only one type of genotype, namely, TT (204 bp) was observed in population. The frequency of TT genotypes was 100% in all screened samples with T allele (1.0). The results revealed that SPP1 T allele seems to be fixed in screened cattle population. Consequently, we could not perform the association study of this substitution with milk production traits

Olecranon Osteotomy Approach for Open Reduction Internal Fixation Analysis of 24 Cases of Fracture Distal Humerus

Background: This study was done for the analysis of the functional outcomes of distal humerus fractures managed by open reduction internal fixation by reviewing 24 cases of fractures of distal humerus which were surgically managed by olecranon osteotomy approach during December 2012 to September 2016. Methods: 16 male patients and 8 female patients with a mean age of 38.12±15.06 years were included in this study. A mean follow up time of 10.2months (range 3-18 months) was done. Flexion, extension, range of motion,mayo elbow performance score (MEPS),disability of shoulder arm and hand score (DASH SCORE), duration of surgery and blood loss were used to assess the functional outcome of fractures of distal humerus managed by open reduction internal fixation using the olecranon osteotomy approach. Results: According to AO foundation (AO) Classification there were no cases of type A or type B , 5 cases of type C1 , 6 cases of type C2 and 13 cases of type C3 fractures.Out of 24 patients9(37.5%), 9(37.5%), 6(25%) obtained Excellent , Good , Fair MEP score respectively. Conclusion:No patient fell under poor category of MEP score.