Molecular typing of mealybug Phenacoccus solenopsis populations from different hosts and locations in Punjab, India.

True identity and existence of genetic variability in mealy bug from different regions holds immense significance for adopting appropriate control measures along with predicting the development of any biotypes. Mealy bug, Phenacoccus solenopsis adults were collected from four host plants i.e., Gossypium hirsutum – cotton (C), Abelmoschus esculentus – okra (O), Pennisetum glaucum – Napier Bajra (B) and a weed – Parthenium hysterophorus (P) in five cotton growing districts i.e., Abohar, Bathinda, Mansa, Muktsar and Faridkot of Punjab state. Variability among different populations was investigated through comparative analysis of four different RAPD markers. The genetic similarity dendrogram established that irrespective of the host plant and the collection site, 20 mealybug populations were distinguishable into two major clades that were related to each other by 68 %. Clade 1 included populations from Abohar district; it also included a single population each from Muktsar and Bathinda districts. The populations from all the other districts were grouped under Clade 2 with genetic similarity of 78 % Even under Clade 2, individual populations appeared to exist in location specific sub-clades. Thus, there is great possibility of development of biotypes which may differ in resistance to insecticides and host plant specificity.

Giant hemicranial calcified subdural empyema - unusual complication following ventriculoperitoneal shunt insertion.

The authors describe an extremely unusual case of a giant hemicranial subdural empyema occurring nine years after insertion of a venticuloperitoneal shunt. Though the empyema was evacuated, the child suffered significant morbidity and remained hemiparetic. The present case highlights the delayed morbidity following a ventriculoperitoneal shunt insertion and the need of prolonged and regular follow up in children who have undergone this procedure.

Surveillance of cold chain system during intensified pulse polio programme--2006 in Chandigarh.

OBJECTIVE: To evaluate the maintenance of cold chain system in the Intensive Pulse Polio Immunization (IPPI) campaign in the Union Territory, Chandigarh. METHODS: A cross sectional study was conducted in 18 designated vaccine sub-depots, where OPV vials were stored prior to IPPI and 25 IPPI booths out of the designated 406 IPPI booths in U.T, Chandigarh. The booths were selected by stratified random sampling technique. 25 Vaccine vials, one from each booth were selected and sent for potency testing at Central Research Institute (CRI)-Kasauli. RESULTS: All the randomly selected vaccine samples were reported portent, as per the reports provided by CRI-Kasauli. Cold chain maintenance and temperature charting was found satisfactory. CONCLUSION: There are some avoidable errors which leaves room for improvement, which can be in the form of provision of adequate number of exhaust fan, voltage stabilizers, etc. Uninterrupted power supply should be ensured. Lastly, more emphasis is to be given on maintenance of cold chain system in reorientation training program of all health functionaries.