RESUMO
Microalgae have been known for broad spectrum of ecological and industrial applications. However, continuous mass culturing of microalgae is still a challenging task. In this context, studies on optimization of culturing conditions like photoperiod and light intensity on nutrients consumption are not uncommon. In the present study, we tried to evaluate the effect of different photoperiod (PP) and photosynthetic photon flux intensity (PPFI) on the nutrient consumption and algal growth of Picochlorum maculatum in shrimp culture effluent. The experiment lasted for 15 days at five different PP cycles (24:0, 18:06, 14:10, 10:14 and 6:18 h light:dark) and four PPFI (50, 100, 150 and 200 µmol m-2 s-1) at 28ºC temperature. Every five days the samples were analyzed and results showed that the PP of 18 h light and 6 h dark and 150 µmol m-2 s-1 of PPFI favouring the growth of P. maculatum with a maximum nutrients consumption (PO43+ 91%, NO3- 68%, NO2- 64%, NH3+ 77%), maximum growth (0.876±0.001 abs) and biomass production (9.93±0.19 g L−1) with highest yield of 9.2% total lipid. Among the photoperiod and photosynthetic photon flux intensity tested, 18:6 h light:dark and 150 µmol m-2 s-1 showed the highest nutrients removal and algal growth. This study strongly suggests that P. maculatum PSDK01 was a potential candidate for further commercial development in larger scale
RESUMO
Background: Dysfunctional apoptosis has an important role in the development of several skin diseases. Psoriatic keratinocytes possess an enhanced ability to resist apoptosis, which might be one of the key pathogenetic mechanisms in psoriasis. P53 and bcl‑2 are two proteins which control apoptosis. Several studies have evaluated the expression of these two proteins in the psoriatic skin, but the results are controversial. Methods: Fifty‑eight cases of psoriatic skin biopsies were studied, and the grade of p53 and bcl‑2 immunostaining was correlated with the histopathological indices of severity. Results: Bcl‑2 expression in the epidermis strongly correlated with the expression in the basal cells and lymphocytes (P – 0.001 and 0.035). There was no correlation with epidermal hyperplasia or with p53 expression in the three compartments. Bcl‑2 expression in the basal layer correlated with the p53 expression in the epidermis (P – 0.027), basal layer (P – 0.015) and the lymphocytes (P – 0.034). There was a strong correlation among the p53 expression in all the compartments. There was also a weak correlation of the p53 expression in the epidermis with the epidermal hyperplasia (P – 0.042). Conclusions: Bcl‑2 does not appear to play an important role in the apoptotic process in psoriasis. In contrast, it is likely that p53 has a far more important role to play. Mutation analysis of the p53 protein is necessary to evaluate if the protein has mutated or if it is of the wild type.
RESUMO
The aim of this study was to assess the open pond and groundwater quality of Tiruchirapalli city of Tamil Nadu, India. The groundwater quality viz., pH, electrical conductivity, total hardness, calcium ion, magnesium ion, chloride, carbonate, bicarbonate, inorganic nitrate, nitrite, phosphate, ammonia and reactive silicate were analysed with respect to various seasons and recorded in the range of 7.1 to 8.1, 97.67 to 533.67 mhos cm-1, 7.07 to 186 mg l-1, 4.67 and 112.0 mg l-1, 2.40 to 92.80 mg l-1, 15.23 to 661.73 mg l-1, 60 to 480 mg l-1, 22.7 to 544.9 mg l-1, 15.33 to 68.00 mg l-1, 0.001 to 0.480 mg l-1, 0.01 to 0.42 mg l-1, 0.02 to 0.75 mg l-1 and 1.1 to 2.96 mg l-1 respectively. The present findings concluded that the quality of ground waters can be considered suitable for human consumption. But the pond water available in and around Tiruchirappalli city was not fit for human usage, agricultural or industrial purposes.