RESUMO
HELLP syndrome is described as a special group of severe preeclampsia characterized by haemolysis (H), elevated liver enzymes (EL) and low platelets (PL), whereas preeclampsia is a disease of unknown etiology characterized by hypertension and proteinuria after the 20th week of gestation. The objective of the study was to evaluate the level of hepcidin in the serum of the patient suffering from HELLP syndrome and preeclampsia. It was done by competitive sandwich ELISA which assesses the serum level of hepcidin in the forty patients suffering from preeclampsia and HELLP syndrome (as cases) and forty healthy individuals (as control). The results of our study showed that serum hepcidin levels were significantly increased among the cases (P =0.00171) in comparison to control (P <0.05) and transferrin saturation levels were significantly decreased (P <0.05) among the cases (P =0.001) in comparison to controls. The decrease in transferrin saturation indicates that there is low serum iron level among the cases. It was concluded that increased hepcidin level may be the cause of anemia among the cases.
RESUMO
Management of cardiovascular risk factors in diabetes demands special attention due to their co-existence. Pioglitazone (PIO) and telmisartan (TLM) combination can be beneficial in effective control of cardiovascular complication in diabetes.In this research,we developed and validated a high throughput LC–MS/MS method for simultaneous quantitation of PIO and TLM in rat plasma. This developed method is more sensitive and can quantitate the analytes in relatively shorter period of time compared to the previously reported methods for their individual quantification. Moreover, till date, there is no bioanalytical method available to simultaneously quantitate PIO and TLM in a single run. The method was validated according to the USFDA guidelines for bioanalytical method validation.A linear response of the analytes was observed over the range of 0.005–10μg/mL with satisfactory precision and accuracy. Accuracy at four quality control levels was within 94.27%–106.10%. The intra-and inter-day precision ranged from 2.32% to 10.14% and 5.02% to 8.12%,respectively.The method was reproducible and sensitive enough to quantitate PIO and TLM in rat plasma samples of a preclinical pharmacokinetic study.Due to the potential of PIO-TLM combination to be therapeutically explored,this method is expected to have significant usefulness in future.
RESUMO
Filariasis is a major public health problem in many tropics and subtropics of Africa, Western Pacific and parts of the Americas, affecting over 83 countries. It is also a public health problem in India. A large majority of the cases found in India are attributed to infection by Wuchereria bancrofti which mainly affects the lymph nodes and the lymphatic channels. Breast is an unusual site for filariasis. In the present case report the patient presented with a gradually increasing breast lump, clinically diagnosed as fibroadenoma