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1.
National Journal of Andrology ; (12): 1075-1079, 2007.
Artigo em Chinês | WPRIM | ID: wpr-232011

RESUMO

<p><b>OBJECTIVE</b>To study the infection of human cytomegalovirus (HCMV) and herpes simplex virus type II (HSV-I) and the morphological characteristics of the infected spermatogenic cells in the semen of infertile men.</p><p><b>METHODS</b>We washed and concentrated the spermatogenic cells obtained from 83 semen samples of infertile men, extracted DNA and then screened HCMV and HSV-II by polymerase chain reaction (PCR). Immunocytochemistry (ICC) was used to detect the expression of correlative virus antigens of the positive semen cells, and the cytology smear was employed to observe the morphological changes of the spermatogenic cells under the microscope after cytology staining.</p><p><b>RESULTS</b>Of all the semen samples, 8 were HCMV positive, 4 HSV-II positive, but none were both HCMV and HSV-II positive. HCMV late antigens were positively and HCMV early antigens negatively expressed in the spermatogenic cells of the 8 HCMV positive cases. In the 4 HSV-II positive cases, 3 were positively and 1 weakly positively expressed. In the semen of the 12 positive cases were found large numbers of immature spermatogenic cells, with different manifestations of apoptosis, such as chromatin pycnosis, vacuoles, damaged nuclear membrane, and apoptotic bodies, but without virus infection-induced specific morphological alteration. Sperm concentration of the positive group was significantly lower than that of the negative (P < 0. 05).</p><p><b>CONCLUSION</b>Spermatogenic cells infected by HCMV and HSV-II may cause pathologic lesions and affect spermatogenesis. Morphologically, the infected spermatogenic cells may undergo some pathologic alteration, such as apoptosis. The rate of HCMV infection is higher among infertile males with pathologic cells in the semen.</p>


Assuntos
Adulto , Humanos , Masculino , Antígenos Virais , Citomegalovirus , Genética , Alergia e Imunologia , Infecções por Citomegalovirus , Patologia , Virologia , DNA Viral , Genética , Herpes Simples , Patologia , Virologia , Herpesvirus Humano 2 , Genética , Alergia e Imunologia , Imuno-Histoquímica , Infertilidade Masculina , Patologia , Virologia , Reação em Cadeia da Polimerase , Sêmen , Biologia Celular , Virologia , Espermatozoides , Biologia Celular , Virologia
2.
Chinese Journal of Pediatrics ; (12): 511-515, 2004.
Artigo em Chinês | WPRIM | ID: wpr-340280

RESUMO

<p><b>OBJECTIVE</b>To explore the expression of vascular endothelial growth factor (VEGF) and its receptors (flt-1 and flk-1) in the retina of retinopathy of prematurity (ROP), and its relation to the alteration of retinal blood vessels.</p><p><b>METHODS</b>Eighty-six newborn Sprague-Dawley rats were randomly divided into hyperoxia and air groups, then each group was further divided into 1, 3, 7 and 14 days subgroups. The rats in hyperoxia group inhaled 75% oxygen and ROP model was thus set up. These animals were sacrificed respectively after 1, 3, 7 and 14 days, then the retinal endothelial cells were marked by CD34 to observe the change of retinal blood vessels. The expression of VEGF, flt-1 and flk-1 in the retina was measured by immunohistochemistry.</p><p><b>RESULTS</b>The retinal capillary density index (RCDI) in control group increased as days went on (F = 21.589, P < 0.01, but it was the least on the 7th day in hyperoxia group, after the rats had been returned to air for 7 days, RCDI increased significantly (F = 67.885, P < 0.01); In the control group, the expression of VEGF and flk-1 was the strongest in the retina on the 7th day, the result had significant difference as compared with the 1st and 14th day (P < 0.05). The expression of VEGF and flk-1 on the 7th day in hyperoxia group was weaker than that of control group (P < 0.05). But on the 14th day in hyperoxia group, they were stronger than that of control (P < 0.05). The localization of the expression of flt-1 was changed when blood vessels altered, but there was no significant difference in expression intensity as a whole (P > 0.05).</p><p><b>CONCLUSION</b>When the premature retina was exposed to hyperoxia, the expression of VEGF and flk-1 was reduced, and retinal blood vessels were also decreased; but the expression of VEGF and flk-1 was stronger in retina when premature rats were exposed to relative hypoxia, and the retinal blood vessels also increased significantly. It is concluded that VEGF and flk-1 may play important roles in the development of retinal blood vessels and its change in ROP. However, flt-1 has less effect compared with flk-1.</p>


Assuntos
Animais , Feminino , Masculino , Ratos , Animais Recém-Nascidos , Modelos Animais de Doenças , Hipóxia , Imuno-Histoquímica , Ratos Sprague-Dawley , Receptores de Fatores de Crescimento do Endotélio Vascular , Retina , Química , Patologia , Doenças Retinianas , Metabolismo , Patologia , Fator A de Crescimento do Endotélio Vascular
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