RESUMO
In a previous search for the differentially expressed genes in keratinocyte differentiation, we identified neutrophil gelatinase-associated lipocalin (NGAL) as a calcium- induced gene. In this study, we further verified the expression of NGAL in cultured keratinocytes as well as in several skin diseases. Reverse transcription-polymerase chain reaction (RT-PCR), Western blot, and ELISA clearly showed that NGAL expression was markedly increased in calcium-induced keratinocyte differentiation in vitro. However, in our previous report, NGAL expression was not detected in normal skin tissue except for hair follicle by in situ hybridization and immunohistochemistry, indicating the difference of cell status between in vitro and in vitro conditions. Interestingly, NGAL expression was highly increased in psoriasis-like inflammatory disorders (lichen planus and pityriasis rubura pilaris) and skin cancers (keratoacanthoma and squamous cell carcinoma), implying that NGAL may be related with the epidermal hyperplasia. Collectively, these results reveal the potential importance of NGAL in the maintenance of skin homeostasis.
Assuntos
Humanos , Proteínas de Fase Aguda/biossíntese , Cálcio/metabolismo , Diferenciação Celular , Meios de Cultura , Meios de Cultivo Condicionados , Ensaio de Imunoadsorção Enzimática , Regulação da Expressão Gênica , Homeostase , Queratinócitos/enzimologia , Lipocalinas/biossíntese , Modelos Biológicos , Proteínas Proto-Oncogênicas/biossíntese , Psoríase/enzimologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Pele/metabolismo , Neoplasias Cutâneas/enzimologiaRESUMO
Genital leiomyoma is a benign tumor that arises from the dartos muscles of the genitalia, areola, and nipple. Leiomyoma of the nipple is the less frequent type of genital leiomyoma. We report a case of genital leiomyoma on the nipple of a 30-year-old man. About 3 years prior to presentation, his left nipple had enlarged slowly and he had felt intermittent paroxysmal pain upon touch. The histopathologic findings showed typical leiomyoma.
Assuntos
Adulto , Humanos , Genitália , Leiomioma , Músculos , MamilosRESUMO
Blue nevi are usually solitary, but they may be multiple and form a plaque composed of multiple papules or nodules with an intervening flat area of blue coloration. It looks like a benign and asymptomatic lesion. However, because of its rarity, no definitive prognosis can be given. An 18-year-old male presented with a 5 x 4 cm-sized, speckled blue-black tumor on his posterior neck, which had existed since birth. Histopathologic examination showed clumps of melanocytes intermingled with irregularly arranged collagen bundles in the mid and lower dermis, with some extending to the subcutaneous tissue. We diagnosed the lesion as a plaque-type blue nevus, based on histopathologic findings of common blue nevus.
Assuntos
Adolescente , Humanos , Masculino , Colágeno , Derme , Melanócitos , Pescoço , Nevo Azul , Parto , Prognóstico , Tela SubcutâneaRESUMO
The most common form of allergic dermatitis due to orthopedic implant is persistent eczematous reaction. We present an allergic dermatitis occurring in the right upper arm of 77-year-old man who presented an erythematous edematous patch & plaque on the right upper arm after humerus osteosynthesis with orthopedic implant. The lesion disappeared after the removal of the orthopedic implant.
Assuntos
Idoso , Humanos , Braço , Dermatite , Úmero , Ortopedia , Testes do EmplastroRESUMO
Low dose finasteride(1mg/d, Propecia(R)) has been widely used as an effective treatment for male androgenetic alopecia. Finasteride is an inhibitor of 5-alpha-reductase, the enzyme responsible for testosterone metabolism to dihydrotestosteron(DHT). We report a case of painful, unilateral idiopathic gynecomastia aggravated by low dose finasteride for male androgenetic alpopecia. A 23-year-old man started treatment with Propecia(R), 1 mg per day. Two months later he presented with a painful enlargement of his right breast. Treatment was stopped and two months later the clinical manifestration had disappeared.
Assuntos
Humanos , Masculino , Adulto Jovem , Alopecia , Mama , Finasterida , Ginecomastia , Metabolismo , TestosteronaRESUMO
No abstract available.
Assuntos
Classificação , Doenças de von Willebrand , Fator de von WillebrandRESUMO
No abstract available.
Assuntos
Coagulação Intravascular Disseminada , Plasma , Tifo por ÁcarosRESUMO
In the previous studies, we found that lumbrokinase an, extract from Korean earthworm, had a strong in vitro thrombolytic effect, and that when added to thrombin had a significant effect in reducing fatality rate in thrombin-induced lung infarction mice model. To determine whether it also has in vivo thrombolytic effect in cerebral embolism model using 9 Spraw Dawly male rats of 200 to 300gm, we cannulated the extemal carotid artery lene tubes and occluded the pterygopalatine arteries. Via the extemal carotid artery, we injected 1 mm3 of human blood clots which were previously mixed with Tc-99m sulfur colloid. After confirming the intracranially situated clot by camera, we injected 3cc of following fiuids to each group of three rats: saline, urokinase, lumbrokinase fraction m Then using Gamma camera of 64*64 m obtained for 1 minute in every 30 minutes. After 150 minutes radioactivities of the clots in the brain were 3.02%, 21 02% urokinase, and lumbrokinase treated animals respectively. In the liver, the uptake of radioactivities was accordingly increased. Brain sections showed no Significant intracranial bleeding in any of the treated animals. Therefore, we conclude that lumbrokinase has in vivo thromboembolism model without producing significant intracranial bleeding. However, compared with its in vitro effects the in vivo effects appear to be less potent. Futher experiments with better designed animal models are warranted.
Assuntos
Animais , Humanos , Masculino , Camundongos , Ratos , Artérias , Encéfalo , Artérias Carótidas , Câmaras gama , Hemorragia , Infarto , Embolia Intracraniana , Fígado , Pulmão , Modelos Animais , Oligoquetos , Radioatividade , Coloide de Enxofre Marcado com Tecnécio Tc 99m , Trombina , Tromboembolia , Ativador de Plasminogênio Tipo UroquinaseRESUMO
Twenty male patients with Korean hemorrhagic fever were evaluated with thrombelastography (TEG) to assess the changes in coagulation system, and the results were compared with those of conventional coagulation tests. Procoagulant activity in the plasma was determined by comparing the reaction time "r" of the normal plasma and that of the mixture of equal parts of the normal plasma and the patient's plasma. The TEG was found to be a useful measure of the changes in the coagulation profile, and provided instant accurate assessment of the patient's hemostatic function. Presence of the procoagulant activity was demonstrated in the plasma of the patients and indicated occurrence of active intravascular coagulation during the early stage of the disease.